Integrase diversity and transcription of the maize retrotransposon Grande

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Abstract:

Grande is an abundant gypsy-like retrotransposon present in the genera Zea and Tripsacum. Related retro transposon families can be found in sorghum, rice, and barley, but not in wheat or rye. We have amplified and sequenced several copies of part of the integrase domain derived from the Zea mays, Zea diploperennis, and Tripsacum dactyloides genomes. There are no significant differences in divergence or clustering between the integrase sequences of these species. The substitution rate for synonimous sites was found to be higher than those of non-synomymous sites; this indicates that Grande integrase has been under purifying selection for function. Grande is transcribed in leaves. The transcripts show sequence diversity similar to that of genomic sequences, but belong to restricted clades; this indicates that only some evolutionary branches of Grande have retained transcriptional competence.Key words: maize, plant genome, retrotransposon, Zea diploperennis, Zea genus.

L'élément Grande est un rétrotransposon de type gypsy qui est abondant chez les genres Zea et Tripsacum. Des familles apparentées de rétrotransposons sont présentes chez le sorgho, le riz et l'orge, mais pas chez le blé ou le seigle. Les auteurs ont amplifié et séquencé plusieurs copies d'une partie du domaine intégrase chez les génomes du Zea mays, du Zea diploperennis et du Tripsacum dactyloides. Il n'y a pas de différences significatives quant à la divergence ou au regroupement entre les séquences d'intégrase chez ces espèces. Le taux moyen de substitutions synonymes plus élevé que celui de substitutions non-synonymes indique que l'intégrase de Grande a subi une sélection purificatrice pour sa fonction. L'élément Grande est transcrit dans les feuilles. Les transcrits présentent une diversité nucléotidique semblable à celle observée chez les séquences génomiques, mais appartiennent à des clades restreints, ce qui indique que seules certaines branches évolutives de l'élément Grande ont conservé la capacité d'être transcrites.Mots clés : maïs, génome de plante, rétrotransposon, Zea diploperennis, genre Zea.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: May 1, 2006

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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