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Development of a wide population of chromosome single-segment substitution lines in the genetic background of an elite cultivar of rice (Oryza sativa L.)

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Abstract:

Naturally occurring allelic variations underlying complex traits are useful resources for the functional analysis of plant genes. To facilitate the genetic analysis of complex traits and the use of marker-assisted breeding in rice, we developed a wide population consisting of 217 chromosome single-segment substitution lines (SSSLs) using Oryza sativa L. 'Hua-Jing-Xian74' (HJX74), an elite Indica cultivar, as recipient, and 6 other accessions, including 2 Indica and 4 Japonica, as donors. Each SSSL contains a single substituted chromosome segment derived from 1 of the 6 donors in the genetic background of HJX74. The total size of the substituted segments in the SSSL population was 4695.0 cM, which was 3.1 times that of rice genome. To evaluate the potential application of these SSSLs for quantitative trait loci detection, phenotypic variations of the quantitative traits of days to heading and grain length in the population consisting of 210 SSSLs were observed under natural environmental conditions. The results demonstrated that there was a wide range of phenotypic variation in the traits in the SSSL population. These genetic materials will be powerful tools to dissect complex traits into a set of monogenic loci and to assign phenotypic values to different alleles at the locus of interest.Key words: rice, mapping population, single segment substitution lines, marker-assisted selection, quantitative trait loci.

La variation allélique naturelle sous-jacente à des caractères complexes constitue une ressource utile pour l'analyse fonctionnelle des gènes. Pour faciliter l'analyse génétique de caractères complexes ainsi que la sélection assistée de marqueurs chez le riz, les auteurs ont développé une population de 217 lignées à simples segments substitués (SSSL) à partir de Oryza sativa L. 'Hua-Jing-Xian74' (HJX74), un cultivar élite de type Indica, comme génotype receveur et 6 autres accessions comme donneurs (dont 2 Indica et 4 Japonica). Chaque SSSL contient un seul segment chromosomique substitué provenant de l'un des 6 riz donneurs introduit dans le génome de HJX74. La taille des segments substitués chez la collection de SSSL totalise 4 695,0 cM, soit 3,1 fois le génome du riz. Afin d'évaluer les applications potentielles de ces SSSL pour des fins de détection de QTL, la variation phénotypique pour le nombre de jours à l'épiaison et la longueur des grains, 2 caractères quantitatifs, a été observée sous des conditions naturelles chez une population comprenant 210 SSSL. Les résultats ont démontré une grande variation phénotypique pour ces caractères au sein de la population de lignées SSSL. Ces ressources génétiques constitueront des outils puissants pour disséquer des caractères complexes en locus simples et pour assigner une valeur phénotypique aux différents allèles aux locus d'intérêt.Mots clés : riz, population de cartographie, lignées à simples segments substitués, sélection assistée de marqueurs, locus de caractères quantitatifs.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: May 1, 2006

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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