Authors: Singh, Dharam; Ahuja, Paramvir S.

Source: Genome, Volume 49, Number 1, January 2006 , pp. 91-96(6)

Publisher: NRC Research Press

Abstract:

Variability in the organization of repeats of 5S rDNA is useful for phylogenetic studies in various crops. We found variable repeats of 5S rDNA gene in the genome of tea (Camellia sinensis (L.) O. Kuntze) during Southern hybridization. Variability in the repeats of 5S rDNA with specific restriction endonuleases (Sau3AI, BamHI, and ApoI) was analyzed in 28 different tea clones representing 3 types of tea. Our results clearly show that the 5S rDNA gene in tea could be used as a molecular marker to distinguish C. sinensis Chinary tea from the other important types of tea, namely Assamica and Cambod. Upon analysis with restriction endonucleases, the 5S rDNA gene in the tea genome was found to be heavily methylated.Key words: Camellia sinensis, 5S rDNA, DNA methylation, restriction endonucleases, molecular marker.

La variabilité dans l'organisation des répétitions d'ADNr 5S s'avère utile pour les études phylogénétiques chez diverses espèces cultivées. Les auteurs ont observé de la variabilité au sein des ADNr 5S chez le théier au cours d'hybridations Southern. Cette variabilité au sein des ADNr 5S, observée avec des enzymes de restriction spécifiques (Sau3AI, BamHI et ApoI), a été examinée chez 28 clones du théier appartenant à 3 types. Les résultats montrent clairement que le gène codant pour l'ADNr 5S du théier pourrait servir de marqueur moléculaire pour distinguer le C. sinensis type Chinary des autres types importantes, Assamica et Cambod. Des analyses de restriction ont révélé que le gène codant pour l'ADNr 5S était fortement méthylé dans le génome du théier.Mots clés : Camellia sinensis, ADNr 5S, méthylation de l'ADN, endonucléases de restriction, marqueur moléculaire.[Traduit par la Rédaction]

Document Type: Research article

Publication date: 2006-01-01

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• From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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