Authors: Barzotti, Rita; Pelliccia, Franca; Rocchi, Angela

Source: Genome, Volume 49, Number 1, January 2006 , pp. 87-90(4)

Publisher: NRC Research Press

Abstract:

In this work, we used antibodies against histone H3 trimethylated at lysine 9 (H3K9m3); against histone H4 acetylated at lysines 5, 8, 12, and 16 (H4ac); and against DNA methylated at 5C cytosine (m5C) to study the presence and distribution of these markers in the genome of the isopod crustacean Asellus aquaticus. The use of these 3 antibodies to immunolabel spermatogonial metaphases yields reproducible patterns on the chromosomes of this crustacean. The X and Y chromosomes present an identical banding pattern with each of the antibodies. The heterochromatic telo meric regions and the centromeric regions are rich in H3K9m3, but depleted in m5C and H4ac. Thus, m5C does not seem to be required to stabilize the silence of these regions in this organism.Key words: DNA methylation, H3 methylation, H4 acetylation, crustacean, Asellus aquaticus.

Les auteurs ont employé des anticorps contre l'histone H3 triméthylée sur la lysine 9 (H3K9m3); contre l'histone H4 acétylée sur les lysines 5, 8, 12 et 16 (H4ac); ou contre l'ADN méthylé en 5C sur la cytosine (m5C) afin d'étudier la présence et la distribution de ces marqueurs dans le génome du crustacé isopode Asellus aquaticus. L'emploi de trois anticorps pour marquer des métaphases spermatogoniales produit des motifs reproductibles sur les chromo somes de ce crustacé. Les chromosomes X et Y présentent un marquage identique avec chacun des trois anticorps. Les régions télomériques hétérochromatiques et les régions centromériques sont riches en H3K9m3 mais pauvres en m5C et H4ac. Ainsi, le m5C ne semble pas être requis pour stabiliser l'inactivation de ces régions au sein de cet organisme.Mots clés : méthylation de l'ADN, méthylation H3, acétylation H4, crustacé, Asellus aquaticus.[Traduit par la Rédaction]

Document Type: Research article

Publication date: 2006-01-01

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• From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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