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High degree of conservation of nuclear microsatellite loci in the genus Clusia

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Abstract:

In plants, microsatellites and their flanking DNA are rarely conserved across a whole genus, let alone other genera in the same family. Therefore, the possibility of using microsatellite primers developed for a species across a large number of plant species in the same genus is often limited. Remarkably, dinucleotide nuclear microsatellites developed for Clusia minor and for Clusia nemorosa amplified homologous microsatellites in species across the whole genus Clusia. In this present study, we report on the DNA sequence variation across the genus of 3 microsatellite loci with varying levels of variation. Compared over the species, there was a correlation between the lengths of the microsatellite loci. Interrupts occurred multiple times and did not seem to lead to the death of the microsatellite. These highly conserved markers will be useful for studying the variable reproductive systems in the genus Clusia.Key words: microsatellite, Clusia, cross-species amplification, microsatellite evolution.

Chez les plantes, les microsatellites et les séquences qui les bordent sont rarement conservés au sein d'un genre et encore moins chez différents genres au sein de la même famille. Ainsi, les possibilités d'exploiter des amorces développées chez une espèce au sein d'une gamme d'espèces appartenant au même genre sont souvent limitées. De façon remarquable, les microsatellites nucléaires dinucléotidiques développés pour le Clusia minor et le Clusia nemorosa amplifient des microsatellites homologues chez d'autres espèces à travers tout le genre Clusia. Les auteurs rapportent ici la variation nucléotidique pour 3 locus microsatellites présentant divers niveaux de variation au sein de ce genre. En comparant les diverses espèces, une corrélation a été notée entre les longueurs des microsatellites. Des interruptions ont été observées à de nombreuses reprises, mais sans que cela mène à la mort du microsatellite. Ces marqueurs hautement conservés seront utiles pour étudier la remarquable variabilité des systèmes reproductifs au sein du genre Clusia.Mots clés : microsatellites, Clusia, amplification interspécifique, évolution des microsatellites.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: October 1, 2005

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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nrc/gen/2005/00000048/00000005/art00021
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