Retrotransposon-microsatellite amplified polymorphism (REMAP) markers for genetic diversity assessment of the rice blast pathogen (Magnaporthe grisea)

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Abstract:

This present study is the first report of the application of the retrotransposon-microsatellite amplified polymorphism (REMAP) technique in fungi. Genome fingerprinting has a major role in the characterization of population structure and in the analysis of the variability in fungi. Retrotransposon-microsatellite amplified polymorphism assay was used in virulent isolates of a rice blast pathogen (Magnaporthe grisea) as a new assay system for genetic variability studies that overcomes the limitations of previous techniques. The high polymorphism observed in REMAP could be due to past or recent actions of retrotransposon in M. grisea. Retrotransposon-microsatellite amplified polymorphism, with its superior marker utility, was concluded to be the marker of choice for characterizing M. grisea isolates.Key words: Retrotransposon-microsatellite amplified polymorphism (REMAP), MAGGY, rice blast, Magnaporthe grisea, genetic diversity, retrotransposons.

La présente étude est la première à rapporter l'utilisation de la technique REMAP chez les champignons. L'établissement d'empreintes génétiques joue un rôle majeur dans la caractérisation de la structure des populations et l'analyse de la variabilité chez les champignons. La technique REMAP a été employée sur des isolats virulents de l'agent pathogène de la pyriculariose du riz (Magnaporthe grisea) afin d'en étudier la variabilité génétique tout en surmontant les limitations inhérentes aux techniques employées précédemment. Le polymorphisme élevé observé pourrait découler des actions passées ou récentes de rétrotransposons chez le M. grisea. La technique REMAP s'est avérée la méthode de choix pour le développement de marqueurs pour des fins de caractérisation d'isolats du M. grisea.Mots clés : REMAP, MAGGY (MAGnaporthe GYpsy-like element), pyriculariose du riz, Magnaporthe grisea, diversité génétique, rétrotransposons.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: October 1, 2005

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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