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Linkage mapping of a dominant male sterility gene Ms-cd1 in Brassica oleracea

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Abstract:

The dominant male sterility gene Ms-cd1 (c, cabbage; d, dominant) was identified as a spontaneous mutation in the spring cabbage line 79-399-3. The Ms-cd1 gene is successfully applied in hybrid seed production of several Brassica oleracea cultivars in China. Amplified fragment length polymorphism (AFLP) technology was used to identify markers linked to the Ms-cd1 gene in bulks of male-sterile and male-fertile individuals of a segregating BC3 population and in a near-isogenic population of 25 male-sterile plants. Twelve markers within a 20-cM interval proximal to the Ms-cd1 gene were identified, 5 of which can be used to select homozygous male-sterile Ms-cd1/ Ms-cd1 plants. Three AFLP markers and 3 sequence characterized amplified region markers that were linked to MS-cd1 mapped onto linkage group O9, corresponding to chromosome 3 of B. oleracea. This region corresponds to the top of chromosome 5 in Arabidopsis thaliana.Key words: Brassica oleracea, dominant male-sterility gene, bulk segregant analysis, amplified fragment length polymorphism (AFLP), genetic mapping.

Un gène dominant de stérilité mâle, Ms-cd1 (c : chou, d : dominant), a été identifié grâce à une mutation spontanée au sein du chou de printemps 79-399-3. Le gène Ms-cd1 est employé avec succès pour la production de semence hybride chez plusieurs cultivars de Brassica oleracea en Chine. À l'aide de la technologie AFLP, des marqueurs liés au gène Ms-cd1 ont été identifiés en comparant des groupes d'individus fertiles ou stériles au sein d'une population BC3 et en exploitant 25 individus stériles d'une population quasi-isogénique. Douze marqueurs situés à moins de 20 cM du gène Ms-cd1 ont été identifiés dont 5 permettent de sélectionner des homozygotes mâles stériles Ms-cd1/Ms-cd1. Trois marqueurs AFLP et 3 marqueurs SCAR liés à Ms-cd1 ont été placés sur le groupe de liaison O9, lequel correspond au chromosome 3 chez B. oleracea. Cette région correspond à la partie supérieure du chromosome 5 chez Arabidopsis thaliana.Mots clés : Brassica oleracea, gène dominant de stérilité mâle, analyse de ségrégation en mélange, AFLP, cartographie génétique.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: October 1, 2005

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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nrc/gen/2005/00000048/00000005/art00010
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