Identification of a new class of recombinant prolamin genes in wheat

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Abstract:

A novel storage protein gene with obvious wheat chimeric structure was isolated from an immature kernel-specific cDNA library prepared from the old Hungarian variety, Bánkúti 1201. This clone contains -gliadin sequences in the 5′ region and LMW-glutenin sequences on the 3′ end. A frameshift mutation was also introduced by the putative recombination event. Hence, the amino acid sequence of the C-terminal region was transformed to a completely new polypeptide. Based on this finding, 7 additional recombinant prolamin genes of similar structure were isolated with specific PCR primers. The 8 chimeric clones seem to be derived from 4 individual -gliadin and 3 LMW-glutenin sequences. These genes show remarkable diversity in size, gliadin:glutenin ratio, frameshift mutations, and sulphur content. The putative functional characteristics of the chimeric polypeptides and problems related to the origin of the encoding genes are discussed.Key words: prolamin, chimeric genes, recombination, wheat cDNA library.

Un nouveau gène codant pour une protéine de réserve et montrant une structure chimérique évidente a été isolé au sein d'une banque d'ADNc spécifique du grain immature préparée à partir d'une vieille variété hongroise, Bánkúti 1201. Ce clone comprend des séquences de type -gliadine en 5′ et des séquences de type gluténine LMW en 3′. Un changement de cadre de lecture a également été introduit suite au possible événement de recombinaison. Ainsi, la séquence peptidique de la région C-terminale est complètement changée. À partir de cette observation, 7 gènes additionnels codant pour des prolamines recombinées de structure semblable ont été isolés à l'aide d'amorces PCR spécifiques. Les 8 clones chimériques semblaient dérivés de 4 -gliadines et de 3 gluténines LMW différentes. Ces gènes montrent une diversité remarquable en matière de taille, de ratio gliadine/gluténine, de changements de cadre de lecture et de contenu en soufre. Les caractéristiques fonctionnelles potentielles de ces polypeptides chimériques et les problèmes liés à la genèse des gènes correspondants sont discutés.Mots clés : prolamine, gènes chimériques, recombinaison, banque d'ADNc de blé.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: October 1, 2005

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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