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Phylogenetic relationships among Secale species revealed by amplified fragment length polymorphisms

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Abstract:

Amplified fragment length polymorphism (AFLP) data were utilized to analyze the phylogenetic relationships among 29 accessions representing 14 of the most commonly recognized ranked species or subspecies in the genus Secale. We observed 789 AFLP markers of 1130 fragments utilizing 18 P-/M- and E-/M- primer combinations. All polymorphic fragments were used to construct phenetic and phylogenetic trees. The resulting phenogram and cladogram had similar tree topologies. Cluster analysis showed that Secale sylvestre was the most distantly related to all other ryes. Annual forms were grouped together, and the perennial forms appeared more closely related to each other. This suggested that life cycle could have played an important role in determining the relationships among Secale species. Secale sylvestre was considered to be the most ancient species, whereas Secale cereale was the most recently evolved species. Amplified fragment length polymorphism analysis clearly separated all Secale species into only 3 major species groups, within the genus Secale: S. sylvestre, Secale montanum (syn. Secale strictum) for perennial forms, and S. cereale for annual forms. This study demonstrated that the AFLP approach is a useful tool for discriminating species differences, and also gave a much better resolution in discerning genetic relationships among Secale species as compared with previous studies using other approaches.Key words: AFLP, Secale, phylogenetic relationship.

Le polymorphisme de longueur des fragments amplifiés (AFLP) a été employé pour analyser les relations phylogénétiques entre 29 accessions représentant 14 des espèces ou sous-espèces les plus communément reconnues au sein du genre Secale. Les auteurs ont obtenu 789 marqueurs AFLP parmi 1130 fragments en employant 18 combinaisons d'amorces P-/M- and E-/M-. Tous les fragments polymorphes ont été employés pour produire des arbres phénétiques et phylogénétiques. Les phénogrammes et dendrogrammes résultants avaient une topologie semblable. Une analyse de groupement a montré que le Secale sylvestre était l'espèce la plus éloignée de tous les autres seigles. Les formes annuelles ont été groupées ensemble et les formes pérennes étaient plus apparentées les unes aux autres. Ceci suggère que le cycle vital pourrait avoir joué un rôle majeur dans l'établissement des relations au sein des espèces du genre Secale. Le S. sylvestre est considéré comme étant l'espèce la plus ancienne, tandis que le Secale cereale serait l'espèce la plus récente. L'analyse AFLP a clairement séparé toutes les espèces entre seulement 3 groupes majeurs : S. sylvestre, Secale montanum (syn. Secale strictum) chez les formes pérennes, et S. cereale chez les formes annuelles. Cette étude montre que les AFLP constituent un outil utile pour discerner les relations génétiques au sein des espèces du genre Secale par comparaison avec les études antérieures faisant appel à d'autres approches.Mots clés : AFLP, Secale, relation phylogénétique.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: October 1, 2005

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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