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Moth sex chromatin probed by comparative genomic hybridization (CGH)

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Abstract:

Comparative genomic hybridization (CGH) with a probe mixture of differently labeled genomic DNA from females and males highlighted the W chromosomes in mitotic plates and the W chromatin in polyploid interphase nuclei of the silkworm Bombyx mori, the flour moth Ephestia kuehniella, and the wax moth Galleria mellonella. The overproportionate fluorescence signal indicated an accumulation of repetitive sequences in the respective W chromosomes. Measurements of the fluorescence signals revealed two components, one that is present also in male DNA (non-W chromosomes) and another one that is present only in or preponderantly in female DNA (W chromosomes). While the W chromosomes of E. kuehniella and G. mellonella had both components, that of B. mori appeared to lack the latter component. Our results show that CGH can be applied to obtain a first estimate of the sequence composition of sex chromosomes in species from which otherwise little is known on the molecular level.Key words: Lepidoptera, comparative genomic hybridization, molecular evolution, W chromosome.

L'hybridation génomique comparée (CGH) à l'aide d'une sonde mixte constituée d'ADN génomiques de mâles ou de femelles et marqués différemment a permis de faire ressortir le chromosome W au sein des plaques mitotiques et la chromatine W dans des noyaux polyploïdes en interphase chez le vers à soie, Bombyx mori, chez la pyrale de la farine, Ephestia kuehniella, et chez la teigne des ruches, Galleria mellonella. L'intensité disproportionnée de la fluorescence indique l'accumulation de séquences répétitives au sein des chromosomes W. Des mesures des signaux fluorescents ont révélé la présence de deux composantes, l'une qui est présente chez les mâles (chromosomes autres que le W) et une autre qui est présente de manière prédominante chez l'ADN femelle (chromosome W). Tandis que les chromosomes W de l'E. kuehniella et du G. mellonella possédaient les deux composantes, celui du B. mori semblait ne pas posséder la seconde. Ces résultats montrent que la CGH peut être employée afin d'obtenir un premier estimé de la composition nucléotidique des chromosomes sexuels chez des espèces pour lesquelles peu de choses sont connues au niveau moléculaire.Mots clés : lépidoptères, hybridation génomique comparée, évolution moléculaire, chromosome W.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2003-04-01

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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