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Molecular cytogenetic analysis of wheat–barley hybrids using genomic in situ hybridization and barley microsatellite markers

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Abstract:

In the present investigation, genomic in situ hybridization (GISH) and barley microsatellite markers were used to analyse the genome constitution of wheat–barley hybrids from two backcross generations (BC1 and BC2). Two BC1 plants carried 3 and 6 barley chromosomes, respectively, according to GISH data. Additional chromosomal fragments were detected using microsatellites. Five BC2 plants possessed complete barley chromosomes or chromosome segments and six BC2 plants did not preserve barley genetic material. Molecular markers revealed segments of the barley genome with the size of one marker only, which probably resulted from recombination between wheat and barley chromosomes. The screening of backcrossed populations from intergeneric hybrids could be effectively conducted using both genomic in situ hybridization and molecular microsatellite markers. GISH images presented a general overview of the genome constitution of the hybrid plants, while microsatellite analysis revealed the genetic identity of the alien chromosomes and chromosomal segments introgressed. These methods were complementary and provided comprehensive information about the genomic constitution of the plants produced.Key words: wheat–barley hybrids, introgressive hybridization, recombination, molecular markers, genomic in situ hybridization (GISH).

L'hybridation génomique in situ (GISH) et des marqueurs microsatellites de l'orge ont été employés afin d'analyser la composition génomique d'hybrides blé–orge de deux générations backcross (BC1 et BC2). Deux plants BC1 étaient porteurs, respectivement, de trois et six chromosomes de l'orge d'après l'analyse GISH. Des fragments chromosomiques additionnels ont été détectés à l'aide des microsatellites. Cinq plants BC2 possédaient des chromosomes complets ou des segments chromosomiques de l'orge. Six plants BC2 ne possédaient aucun matériel génétique en provenance de l'orge. Les marqueurs moléculaires ont révélé la présence de segments du génome de l'orge d'une taille telle qu'un seul marqueur était présent ; une telle situation résultant vraisemblablement de la recombinaison entre les chromosomes du blé et de l'orge. Le criblage de populations backcross issues d'hybridations intergénériques pourrait se faire de manière efficace en employant à la fois l'hybridation génomique in situ et les microsatellites. Les images GISH fournissaient un aperçu général de la composition génomique des hybrides tandis que l'analyse des microsatellites révélait l'identité de la chromatine étrangère et des segments introduits. Ces méthodes étaient complémentaires et fournissaient une description assez complète de la composition génomique des plantes obtenues.Mots clés : hybrides blé–orge, hybridation introgressive, recombinaison, marqueurs moléculaires, hybridation génomique in situ (GISH).[Traduit par la Rédaction]

Document Type: Research Article

Publication date: April 1, 2003

More about this publication?
  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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nrc/gen/2003/00000046/00000002/art00015
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