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The genetic basis of C-glycosyl flavone B-ring modification in maize (Zea mays L.) silks

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Abstract:

Resistance to corn earworm (CEW) (Helicoverpa zea Boddie) has been attributed to high concentrations of C-glycosyl flavones and chlorogenic acid in maize (Zea mays L.) silks. The most common C-glycosyl flavones isolated from maize silks are maysin, apimaysin, and methoxymaysin, which are distinguished by their B-ring substitutions. For a better understanding of the genetic mechanisms underlying the synthesis of these compounds, we conducted a quantitative trait locus (QTL) study with two populations: (Tx501 × NC7A)F2 and (Tx501 × Mp708)F2. For chlorogenic acid, maysin, and methoxymaysin concentration, the major QTL for both populations was located on chromosome 4 near umc1963. For apimaysin, the major QTL in both populations was located at the position of the pr1 locus on chromosome 5. The QTL alleles on chromosome 4 that increased the synthesis of methoxymaysin significantly decreased the synthesis of maysin and chlorogenic acid. This decrease in maysin concentration was four-fold greater than the increase in methoxymaysin. Our results indicate that the QTL on chromosome 4, responsible for the increase in methoxymaysin synthesis, alters the dynamics of both the phenylpropanoid and flavonoid pathways.Key words: pr1, flavonoid 3'-hydroxylase, maysin, apimaysin, methoxymaysin.

La résistance au ver de l'épi du maïs (CEW) (Helicoverpa zea Boddie) a été attribuée à de fortes teneurs en flavones C-glycosylées et en acide chlorogénique dans les soies du maïs (Zea mays L.). Les flavones C-glycoslyées les plus communément extraites des soies du maïs sont la maysine, l'apimaysine et la méthoxymaysine, lesquelles se distinguent les unes des autres par les substitutions au niveau de l'anneau B. Afin de mieux cerner les mécanismes génétiques qui sous-tendent la synthèse de ces composés, les auteurs ont réalisé une étude de locus à caractère quantitatif (QTL) sur deux populations : (Tx501 × NC7A)F2 et (Tx501 × Mp708)F2. En ce qui a trait aux teneurs en acide chlorogénique, en maysine et en méthoxymaysine, un QTL majeur a été identifié sur le chromosome 4 chez les deux populations, à proximité du marqueur umc1963. Pour l'apimaysine, le QTL principal chez les deux populations était situé au locus pr1 sur le chromosome 5. Les allèles du locus QTL situé sur le chromosome 4 et qui accroissaient de manière significative la synthèse de méthoxymaysine diminuaient la synthèse de la maysine et de l'acide chloro génique. Cette diminution de la synthèse de maysine était quatre fois plus considérable que l'accroissement observé pour la méthoxymaysine. Ces résultats indiquent que le locus QTL situé sur le chromosome 4, lequel est responsable d'un accroissement de la synthèse de méthoxymaysine, modifie la dynamique de la voie des phénylpropanoïdes et des flavonoïdes.Mots clés : pr1, flavonoïde 3'-hydroxylase, maysine, apimaysine, méthoxymaysine.

Document Type: Research Article

Publication date: April 1, 2003

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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