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Cytogenetic and molecular characterization of intergeneric hybrids between Brassica napus and Orychophragmus violaceus

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Abstract:

Twenty-two intergeneric hybrids from a cross between Brassica napus (AACC, 2n = 38) cultivar Oro and the ornamental crucifer Orychophragmus violaceus (OO, 2n = 24) were produced without embryo rescue. The plants were classified into three groups based on morphological and cytological observations and RAPD banding patterns. Plants of Group I had morphological traits of both parents and 2n = 29 chromosomes. In these plants, 62.1% of the pollen mother cells (PMCs) had the pairing configuration 1 III + 9 II + 8 I; the remaining PMCs had 10 II + 9 I. The plants possessed 97.6–98.8% B. napus specific and 9.2–11.7% O. violaceus specific RAPD fragments. Plants of Group II exhibited novel morphological traits and possessed 2n = 35, 36, or 37 chromosomes. Plants of Group III were morphologically similar to B. napus and possessed 2n = 19, 37, 38, or 39 chromosomes. Plants of Group II and Group III had 94.1–99.4% B. napus specific RAPD fragments and no O. violaceus specific RAPD fragments. Chromosome fragments were observed in PMCs of most of the F1 plants in all groups. Based on the cytological results and RAPD analysis, it is suggested that genome doubling and chromosome elimination occurred in the intergeneric hybrids of B. napus × O. violaceus.Key words: Brassica, intergeneric hybridization, meiosis, RAPD, chromosome elimination.

Vingt-deux hybrides intergénériques ont été obtenus naturellement à partir d'un croisement entre la variété de Brassica napus (AACC, 2n = 38) Oro et l'espèce horticole Orychophragmus violaceus (OO, 2n = 24). Les hybrides ont été caractérisés par l'étude de leur morphologie, de leur comportement méiotique et de leurs profils RAPD. Les plantes du groupe I (2n = 29) possédaient des caractères morphologiques des deux parents. La majorité (62.1%) des cellules mères du pollen possédaient la configuration méiotique 1 III + 9 II + 8 I et le reste des cellules possédait la configuration 10 II + 9 I. Les plantes du groupe I possédaient 97.6–98.8% des bandes RAPD de B. napus et 9.2–11.7% des bandes d'O. violaceus. Les plantes du groupe II (2n = 35, 36 ou 37) possédaient de nouveaux caractères morphologiques. Les plantes du groupe III (2n = 19, 37, 38 ou 39) étaient morphologiquement semblables à B. napus. Les plantes des groupes II et III possédaient 94.1–99.4% des bandes RAPD de B. napus, mais aucune bande d'O. violaceus. Des fragments chromosomiques ont été observés dans les cellules mères du pollen chez la majorité des hybrides des trois groupes. Les résultats cytologiques et moléculaires suggèrent des phénomènes de duplication génomique et d'élimination chromosomique chez les hybrides issus de ce croisement.Mots clés : Brassica, hybridation intergénérique, méïose, RAPD, élimination chromosomique.

Keywords: Brassica; RAPD; chromosome elimination; hybridation intergénérique; intergeneric hybridization; meiosis; méïose; élimination chromosomique

Document Type: Research Article

Publication date: February 1, 2002

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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nrc/gen/2002/00000045/00000001/art00016
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