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DNA polymorphism in the living fossil Ginkgo biloba from the Eastern United States

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Abstract:

Random amplified polymorphic DNA (RAPD) analysis is a valuable tool in studying inter- and intra-specific genetic variations, patterns of gene expression, and for the identification of specific genes using nearly isogenic variants. Here we used RAPD analysis to study the genetic variation in Ginkgo biloba grown in the eastern United States. Our results support the evidence that Southern blot hybridization of RAPD using probes made from cloned DNA fragments allows a more accurate analysis of the RAPD pattern than dye-stained gels or Southern blot hybridization of RAPD blots using probes made from purified PCR products. Using these techniques, we observed a high degree of relatedness among plants grown in certain localities although significant genetic variation may exist in the species, and could be a possible explanation for the observed variations in the efficacy of medications derived from G. biloba extract.Key words: Ginkgo biloba, Ginkgo extract, RAPD analysis, RFLP analysis.

L'analyse RAPD (ADN polymorphe amplifié au hasard) constitue un outil précieux pour étudier la variation génétique intra- et inter-spécifique, l'expression génique et l'identification de gènes spécifiques à l'aide de variants quasi-isogéniques. Les auteurs ont employé l'analyse RAPD pour étudier la variation génétique au sein du Ginkgo biloba poussant dans l'est des États-Unis. Leurs résultats indiquent que l'hybridation Southern sur des buvardages RAPD au moyen de sondes provenant de fragments d'ADN cloné permet une analyse plus précise des motifs RAPD par rapport à la simple coloration des gels ou à l'hybridation Southern à l'aide de sondes préparées à partir d'amplicons purifiés. À l'aide de ces techniques, les auteurs ont observé un fort degré de parenté parmi les plantes dans certaines localités bien qu'une variation génétique significative peut exister au sein de l'espèce. Cela pourrait constituer une cause possible de la variation observée quant à l'efficacité des produits médicamenteux tirés de l'extrait du G. biloba.Mots clés : Ginkgo biloba, extrait du Ginkgo, analyse RAPD, analyse RFLP.[Traduit par la Rédaction]

Keywords: Ginkgo biloba; Ginkgo extract; RAPD analysis; RFLP analysis; analyse RAPD; analyse RFLP; extrait du Ginkgo

Document Type: Research Article

Publication date: February 1, 2002

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  • From its inception in 1957, this international cytogenetics journal has catered to the research areas of the members of the Genetics Society of Canada; traditionally, these have included agriculture, entomology, genetics/cytogenetics, and evolutionary mechanisms. The contents of the journal have evolved as contributors developed new technologies and interests. A 20-member Editorial Board is composed of scientists from around the world. Reviews and commentary from respected experts are often featured.
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nrc/gen/2002/00000045/00000001/art00003
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