mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataractaPyganodon fragilis freshwater mussel complex in Atlantic Canada

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Two nominal species of freshwater mussels, Pyganodon cataracta (Say, 1817) (eastern floater) and Pyganodon fragilis (Lamarck, 1819) (Newfoundland floater), occur in Atlantic Canada, but their taxonomic status has been controversial. We analyzed the female-transmitted mitochondrial cytochrome c oxidase subunit I (i.e., FCOI) and nuclear internal transcribed spacer region (ITS-1) gene sequences, as well as multilocus amplified fragment length polymorphism (AFLP) markers, to evaluate genetic variation in Pyganodon specimens from Atlantic Canada. We found that Pyganodon samples from this region displayed no or extremely low (i.e., <0.5%) genetic divergence for ITS-1 and FCOI sequence data, respectively. Inferences from nested clade analysis of FCOI haplotypes suggest restricted gene flow with isolation by distance and contiguous range expansion. Analysis of molecular variance inferred from presence or absence of nuclear-encoded AFLP bands showed moderate geographic structuring among provinces but no correspondence between mtDNA haplotypes and AFLP profiles. Similarly, there was no correspondence between presence of a single or a double loop on the umbo and FCOI haplotype. These mtDNA and AFLP markers demonstrate limited genetic differentiation within the Pyganodon cataractaPyganodon fragilis freshwater mussel complex in Atlantic Canada and question the species-level distinctness of these putative taxa.

Keywords: Newfoundland floater; Pyganodon cataracta; Pyganodon fragilis; Unionoida; Unionoïdés; anodonte de Terre-Neuve; anodonte de l’Est; eastern floater; freshwater mussels; moules d’eau douce; phylogeography; phylogéographie; taxinomie; taxonomy

Document Type: Research Article

DOI: http://dx.doi.org/10.1139/z2012-100

Affiliations: 1: Department of Biological Sciences, Kent State University, Kent, OH 44242, USA. 2: Department of Natural Science, New Brunswick Museum, Saint John, NB E2K 1E5, Canada. 3: Nova Scotia Museum of Natural History (Zoology), Halifax, NS B3H 3A6, Canada. 4: Department of Biology, Acadia University, Wolfville, NS B4P 2R6, Canada.

Publication date: November 19, 2012

More about this publication?
  • Published since 1929, this monthly journal reports on primary research contributed by respected international scientists in the broad field of zoology, including behaviour, biochemistry and physiology, developmental biology, ecology, genetics, morphology and ultrastructure, parasitology and pathology, and systematics and evolution. It also invites experts to submit review articles on topics of current interest.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more