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Triacylglyceride (TAG) profiles of integumentary lipids isolated from three bat species determined by matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI–TOF MS)

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Abstract:

Lipids secreted from sebaceous glands in bat integument may play a role in determining host pathogenicity by the fungus Geomyces destructans in white-nose syndrome (WNS). To investigate this, we have determined the triacylglycerol (TAG) profiles for three bat species: eastern red bats (Lasiurus borealis (Müller, 1776)), evening bats (Nycticeius humeralis (Rafinesque, 1818)), and big brown bats (Eptesicus fuscus (Beauvois, 1796)). Neutral lipids extracted from the hair and wing tissue were fractionated by preparative thin-layer chromatography (TLC) into four major lipid bands corresponding to cholesterol, free fatty acids (FFAs), TAGs, and sterol–wax esters. Densitometry showed higher proportions of TAGs in hair than from wing tissue. TAG bands were recovered and analyzed by matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI–TOF MS). Mass spectra showed sodiated TAG species with variable fatty acyl (FA) moieties range from m/z 715.6–911.8. High intensity ion peaks were consistent with 16:0 and 18:1 as dominant FA moieties, and these were identified as palmitic and oleic acids, respectively, by liquid chromatography electrospray ionization mass spectrometry. We determined significant differences in TAG profiles between three bat species by MALDI–TOF MS, providing the first description of integumentary lipids in bats. In this study, we performed the first TAG profiling of bats, which suggest such profiles may be species-specific in bats.

Keywords: Lasiurus borealis; bat integument; chauve-souris rousse; chromatographie sur couche mince; eastern red bat; lipid profiles; matrix assisted laser desorption–ionization time-of-flight mass spectrometry; profils de lipides; spectrométrie de masse à désorption–ionisation par impact laser assistée par matrice et à temps de vol; syndrome du museau blanc; thin-layer chromatography; triglycerides; triglycérides; tégument de chauve-souris; white-nose syndrome

Document Type: Research Article

DOI: http://dx.doi.org/10.1139/z2012-078

Affiliations: 1: Department of Biological Sciences, Arkansas State University, P.O. Box 599, State University, AR 72467, USA. 2: Arkansas Biosciences Institute, Arkansas State University, P.O. Box 639, State University, AR 72467, USA.

Publication date: September 22, 2012

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  • Published since 1929, this monthly journal reports on primary research contributed by respected international scientists in the broad field of zoology, including behaviour, biochemistry and physiology, developmental biology, ecology, genetics, morphology and ultrastructure, parasitology and pathology, and systematics and evolution. It also invites experts to submit review articles on topics of current interest.
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