Sperm morphology, ATP content, and analysis of motility in Atlantic halibut (Hippoglossus hippoglossus)

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Abstract:

Spermatozoon of Atlantic halibut (Hippoglossus hippoglossus (L., 1758)) is uniflagellated, lacks an acrosome, and is differentiated into a head, midpiece, and flagellum. There are two to five mitochondria in the midpiece, as well as proximal and distal centrioles. The flagellum consisted of 9 + 2 microtubules surrounded by plasma membrane, which is extended at the proximal part of the flagellum owing to the presence of vacuoles. After sperm activation in seawater, sperm motility and velocity decreased from 98.4% ± 3.4% and 170.3 ± 8.9 µm·s-1 at 15 s after sperm activation to 4.8% ± 4.7% and 9.2 ± 8.9 µm·s-1 at 120 s after sperm activation, respectively. ATP content (nmol·L-1 ATP per 108 spermatozoa) significantly decreased at 60 s after sperm activation (5.9 ± 1.5) compared with at 0 and 30 s after sperm activation (14.9 ± 1.5 and 14.5 ± 1.5, respectively). Beating waves propagated along the full length of the flagellum after sperm activation, whereas waves were restricted to the proximal section during the latter motility period. Wave amplitude significantly decreased at 45 s after sperm activation, but wavelength did not differ. The present study showed associations among sperm morphology, ATP content, flagellar wave parameters, and sperm velocity, which could be used in comparative spermatology.

Le spermatozoïde du flétan atlantique (Hippoglossus hippoglossus (L., 1758)) est uniflagellé, sans acrosome et différencié en tête, corps médian et flagelle. Il y a deux à cinq mitochondries dans le corps médian, ainsi que des centrioles proximaux et distaux. Le flagelle consiste en 9 + 2 microtubules entourés d’une membrane plasmatique qui se prolonge dans la partie proximale du flagelle à cause de la présence de vacuoles. Après l’activation des spermatozoïdes dans l’eau de mer, la motilité et la vitesse des spermatozoïdes diminuent respectivement de 98,4 % ± 3,4 % et de 170,3 ± 8,9 µm·s-1 à 15 s après l’activation à 4,8 % ± 4,7 % et 9,2 ± 8,9 µm·s-1 à 120 s après l’activation. Le contenu en ATP (nmol·L-1 ATP par 108 spermatozoïdes) diminue significativement 60 s après l’activation (5,9 ± 1,5) par comparaison à 0 et 30 s après l’activation (respectivement 14,9 ± 1,5 et 14,5 ± 1,5). Les ondulations de battement se propagent sur toute la longueur du flagelle après l’activation, mais sont restreintes à la partie proximale durant la période de motilité subséquente. L’amplitude des ondulations diminue significativement à 45 s après l’activation, mais leur longueur d’onde ne change pas. Notre étude met en lumière des associations entre la morphologie des spermatozoïdes, leur contenu en ATP, les variables ondulatoires des flagelles et la vitesse des spermatozoïdes qui pourraient servir en spermatologie comparée.

Document Type: Research Article

Publication date: March 1, 2011

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