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Localization of metallothionein, heat shock protein (Hsp70), and superoxide dismutase expression in Hemidiaptomus roubaui (Copepoda, Crustacea) exposed to cadmium and heat stress

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Immunohistochemical methods were applied in the present study to investigate the expression of stress proteins such as metallothioneins (MT), which are metal-binding proteins, and heat shock proteins (Hsp70), as well as an antioxidant enzyme (superoxide dismutase, SOD), in the freshwater crustacean copepod Hemidiaptomus roubaui (Richard, 1888) exposed to cadmium or heat stress. The results show a tissue-specific distribution of MT-like protein after cadmium exposure in the brain and in the nerve cord. Cadmium stress did not provoke inducible Hsp70 or SOD expression. Unlike cadmium, heat stress induced the expression of Hsp70 and SOD in the shell glands, a structure involved in the reproductive function, and more particularly in the formation of the diapause egg envelope. MT expression is not induced in animals exposed to heat stress.

Dans notre travail, des techniques immunohistochimiques ont été utilisées pour étudier l’expression de protéines de stress, telles que les métallothionéines (MT), qui piègent les métaux lourds, et les protéines de stress thermique (Hsp70), ainsi que d’une enzyme antioxydante (la superoxide dismutase, SOD), chez un crustacé copépode d’eau douce Hemidiaptomus roubaui (Richard, 1888) exposé au cadmium ou à un stress thermique. Les résultats obtenus montrent une distribution spécifique des MT chez H. roubaui dans le cerveau et dans la chaîne nerveuse ventrale après exposition au cadmium. L’exposition au cadmium n’induit pas l’expression des Hsp70 ni de la SOD, contrairement au stress thermique qui provoque leur expression dans les glandes coquillières, une structure impliquée dans la fonction de reproduction, et plus particulièrement dans la formation de l’enveloppe des oeufs de diapause. Les MT ne sont pas exprimées chez les animaux exposés à un stress thermique.

Document Type: Research Article

Publication date: March 1, 2007

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  • Published since 1929, this monthly journal reports on primary research contributed by respected international scientists in the broad field of zoology, including behaviour, biochemistry and physiology, developmental biology, ecology, genetics, morphology and ultrastructure, parasitology and pathology, and systematics and evolution. It also invites experts to submit review articles on topics of current interest.
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