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Oxidative balance and immunodetection of antioxidant enzymes in the hepatopancreas of the crab Chasmagnathus granulata subjected to anoxia and reoxygenation

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Abstract:

The present study shows the activities and concentrations of the antioxidant enzymes superoxide dismutase (SOD) and glutathione S-transferase (GST) in the hepatopancreas of the crab Chasmagnathus granulata (Dana, 1851) (Decapoda, Brachyura) when exposed to periods of anoxia (8h) and aerobic recovery (20 and 40min post anoxia). Exposure to anoxia did not cause any change in the activities of the enzymes analyzed. The activities of these enzymes did not change with a decrease in environmental oxygen concentration. During reoxygenation, enzyme activities returned to control levels. The concentrations and activities of SOD and GST presented different response profiles. Exposure to anoxia caused increased lipoperoxidation (conjugated dienes and thiobarbituric acid reactive substances), whereas aerobic recovery reduced lipoperoxidation. The results of this study showed that C. granulata adjusts its antioxidant defense systems in an attempt to reduce and (or) avoid damage resulting from the reintroduction of oxygen.

Notre étude présente l’activité et les concentrations des enzymes antioxydantes, superoxyde dismutase (SOD) et glutathione S-transférase (GST), dans l’hépatopancréas du crabe Chasmagnathus granulata (Dana, 1851) (Decapoda, Brachyura) lors d’expositions à des périodes d’anoxie (8 h) et de récupération aérobie (20 et 40 min). L’exposition à l’anoxie ne change pas les activités des enzymes examinées. L’activité de ces enzymes ne varie pas en fonction du déclin des concentrations d’oxygène du milieu. Durant la réoxygénation, leur activité retourne au niveau de celle des témoins. Les concentrations et les activités de SOD et de GST présentent des profils de réaction différents. L’exposition à l’anoxie produit une lipoperoxydation accrue (diènes conjugués et substances réactives à l’acide thiobarbiturique), mais la récupération aérobie réduit la lipoperoxydation. Les résultats de notre étude montrent que C.granulata ajuste ses systèmes de défense antioxydante afin d’essayer de réduire et (ou) d’éviter les dommages causés par la réintroduction de l’oxygène.

Document Type: Research Article

Publication date: May 1, 2006

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  • Published since 1929, this monthly journal reports on primary research contributed by respected international scientists in the broad field of zoology, including behaviour, biochemistry and physiology, developmental biology, ecology, genetics, morphology and ultrastructure, parasitology and pathology, and systematics and evolution. It also invites experts to submit review articles on topics of current interest.
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