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Effect of glycoinositolphospholipids on the growth and differentiation of Trypanosoma cruzi subpopulations

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Abstract:

We have analyzed the biological effects of glycoinositolphospholipids (GIPLs) derived from Trypanosoma cruzi Chagas, 1909 isolates (G-645 and C-48) on the behavior of clone Dm 28c and the Y strain during their extracellular development in vitro and in the invertebrate host Rhodnius prolixus Stål, 1859. In vitro, a direct correlation between the presence of GIPLs and proliferation of both Dm 28c and the Y strain was observed. The GIPLs purified from strain G-645 induced higher differences in the levels of growth of clone Dm 28c when compared with GIPLs purified from C-48 or Leptomonas samueli Carvalho, 1973. Differentiation in the presence of G-645 GIPLs was significantly reduced (48 h), while a minor effect was observed with GIPLs from C-48 (72 h). Parasites adhered preferentially to areas on slides coated with GIPLs from G-645 but not GIPLs from C-48. Rhodnius prolixus fed with GIPLs from G-645 or paraformaldehyde-fixed G-645 parasites, coincident with or after establishment of Dm 28c infection, presented different growth and differentiation patterns compared with control bugs. Our data suggest the occurrence of intraspecific cooperation and (or) competition among the different subpopulations that make up a specific T. cruzi strain.

Nous avons analysé les activités biologiques des molécules de glycoinositolphospholipides (GIPL) dérivées d'isolats G-645 et C-48 de Trypanosoma cruzi Chagas, 1909 en relation au comportement du clone Dm 28c et de la souche Y pendant leur développement extra-cellulaire in vitro et dans l'hôte invertébré Rhodnius prolixus Stål, 1859. Il existe in vitro une corrélation directe entre la présence des GIPL et la prolifération tant des Dm 28c que de la souche Y. Les GIPL purifiés à partir de la souche G-645 induisent une croissance plus importante du clone Dm 28c que les GIPL provenant de C-48 ou de Leptomonas samueli Carvalho, 1973. La différenciation en présence des GIPL de G-645 est considérablement réduite (48 h), mais il y a peu d'effet avec les GIPL de C-48 (72 h). L'adhésion des parasites s'observe davantage dans les régions des lames traitées avec les GIPL de G-645 mais pas dans celles traitées avec le C-48. Des R. prolixus, nourris avec des GIPL de G-645 ou des parasites G-645 fixés à la paraformaldéhyde, simultanément à l'établissement d'une infection avec les clones Dm 28c ou après, présentent des profils de croissance et de différenciation distincts de ceux des insectes témoins. Nos résultats laissent croire à l'existence d'une compétition intraspécifique et (ou) d'une coopération parmi les différentes sous-populations qui composent une souche particulière de T. cruzi.

Document Type: Research Article

Publication date: February 1, 2005

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  • Published since 1929, this monthly journal reports on primary research contributed by respected international scientists in the broad field of zoology, including behaviour, biochemistry and physiology, developmental biology, ecology, genetics, morphology and ultrastructure, parasitology and pathology, and systematics and evolution. It also invites experts to submit review articles on topics of current interest.
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