Skip to main content

BmNPV alters NADP-dependent malate dehydrogenase activity and associated macromolecules and retards growth and development of the mulberry silkworm, Bombyx mori L., during the final instar

Buy Article:

$50.00 plus tax (Refund Policy)

Abstract:

Changes in tissue-specific NADP-dependent malate dehydrogenase (MDH) activity and protein and cholesterol contents and retardation of growth and development in the mulberry silkworm, Bombyx mori L., induced by infection with a baculovirus, the B. mori nucleopolyhedrovirus (BmNPV), were investigated. The study revealed that the relative growth rate and development of the fifth-instar larva was significantly inhibited during the progression of nuclear polyhedrosis disease at various times post inoculation (p.i.). Percent pupation and reproductive output were also adversely affected. NADP-dependent MDH activity in the hemolymph peaked sharply at 6 h p.i. and then gradually decreased, reaching a minimum at 264 h p.i., with exceptions at 30 and 72 h p.i. These levels of MDH activity were different from those in non-infected controls. In fat-body tissues, NADP-dependent MDH activity was significantly higher in infected insects than in non-infected controls of the same age. These results indicate that baculovirus infection causes significant changes in intermediary metabolic pathways, causing a significant fall and rise in protein and cholesterol contents in tissues during development of fifth-instar larvae.

Nous avons étudié les changements dans l'activité spécifique aux tissus de la malate déshydrogénase (MDH) liée à NADP, les protéines et les concentrations de cholestérol, de même que les ralentissements dans la croissance et le développement du ver à soieBombyx mori L. à la suite d'infections du baculovirus BmNPV (le nucléopolyédrovirus de B. mori). L'étude a révélé que le taux de croissance relatif et le développement de la larve de 5e stade étaient inhibés significativement pendant la maturation progressive de la polyédrose à divers moments après l'inoculation (p.i.). Le pourcentage d'individus qui ont fait leur nymphose et le rendement de la reproduction étaient également affectés. L'activité de la malate déshydrogénase (NADP) a atteint un sommet important après 6 h dans l'hémolymphe, pour ensuite diminuer graduellement jusqu'à un minimum après 264 h, à deux exceptions près, à 30 et à 72 h. Ces niveaux d'activité de la malate déshydrogénase diffèrent de ceux qu'on enregistre chez des témoins sains. L'activité de la malate déshydrogénase (NADP) est significativement plus élevée dans les corps gras des insectes infectés que dans ceux d'insectes sains. Ces résultats indiquent que les infections de ce baculovirus entraînent des modifications significatives des voies métaboliques intermédiaires, causant tour à tour des montées et des chutes significatives, spécifiques aux tissus, des contenus en protéines et en cholestérol durant le développement de la larve de 5e stade.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: August 1, 2002

More about this publication?
  • Published since 1929, this monthly journal reports on primary research contributed by respected international scientists in the broad field of zoology, including behaviour, biochemistry and physiology, developmental biology, ecology, genetics, morphology and ultrastructure, parasitology and pathology, and systematics and evolution. It also invites experts to submit review articles on topics of current interest.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
nrc/cjz/2002/00000080/00000008/art00014
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more