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Microgeographic variation in allozymes and mitochondrial DNA of Microtus richardsoni, the water vole, in the Beartooth Mountains of Montana and Wyoming, U.S.A.

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Microtus richardsoni, the water vole, is listed as a sensitive species within region 2 of the USDA Forest Service. Because it is a sensitive species, the water vole's ability to disperse becomes an important management concern in terms of its population viability. Both allozyme and mitochondrial DNA analyses were used to study microgeographic population structure within a group of populations from four adjacent watersheds of the Beartooth Mountains of Montana and Wyoming. Of 31 protein loci examined, only ADH, EST-1, and SOD-1 were polymorphic. ADH and EST-1 were in Hardy–Weinberg equilibrium, but SOD-1 was not. Mitochondrial DNA was found to be polymorphic with 9 restriction enzymes. The size of the fragments produced by each restriction enzyme was estimated and ordered into a site map. In total, 51 sites were found with 9 restriction enzymes resulting in 29 different haplotypes from 142 individuals. Shared haplotypes were geographically contiguous except for one. There was no significant difference in genetic distance among water voles from the Beartooth Mountains, and only the outgroup from Togwotee Pass in the Absaroka Mountains of Wyoming had significant genetic distance. This suggests the possibility of dispersal between water voles from the contiguous watersheds of the Beartooth Mountains but not between the Beartooth Mountains and Togwotee Pass populations.

Microtus richardsoni, le campagnol de Richardson, fait partie de la liste des espèces vulnérables de la région 2 du service des forêts de l'USDA. À cause de la sensibilité élevée de ces campagnols, leur capacité de se disperser devient une préoccupation importante pour les gestionnaires qui s'inquiètent de la viabilité des populations. Une analyse des allozymes et une analyse de l'ADN mitochondrial ont permis d'étudier la structure microgéographique de la population dans un groupe de populations de quatre bassins hydrographiques adjacents des montagnes Beartooth au Montana et au Wyoming. De 31 locus protéiniques examinés, seuls ADH, EST-1 et SOD-1 étaient polymophes. ADH et EST-1, mais pas SOD-1, étaient en équilibre Hardy–Weinberg. L'ADN mitochondrial s'est avéré polymorphe à l'utilisation de 9 enzymes de restriction. La taille des fragments produits par chaque enzyme de restriction a été estimée et chaque mesure a été reportée sur une carte géographique. Les 9 enzymes de restriction ont révélé 51 sites au total, soit 29 haplotypes chez 142 individus. Sauf dans un cas, les haplotypes communs sont contigus géographiquement. Il n'y a pas de différence significative de distance génétique chez les campagnols des montagnes Beartooth et seuls les campagnols du groupe externe du col de Togwotee, dans les montagnes Absaroka, au Wyoming, ont une distance génétique significative, ce qui indique la possibilité d'une dispersion chez les campagnols des bassins contigus des monts Beartooth, mais pas des populations des monts Beartooth à celle du col de Togwotee.[Traduit par la Rédaction]

Document Type: Research Article

Publication date: 2001-07-01

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  • Published since 1929, this monthly journal reports on primary research contributed by respected international scientists in the broad field of zoology, including behaviour, biochemistry and physiology, developmental biology, ecology, genetics, morphology and ultrastructure, parasitology and pathology, and systematics and evolution. It also invites experts to submit review articles on topics of current interest.
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