If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Leflunomide induces immunosuppression in collagen-induced arthritis rats by upregulating CD4+CD25+ regulatory T cells

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

This study was to investigate the effect of leflunomide on the immunosuppressive CD4+CD25+ regulatory T cells (CD4+CD25+ Tregs) in collagen-induced arthritis (CIA) rats. CIA was induced by collagen type II in Wistar rats. Immunofluorescence flow cytometry and RT-PCR were used to determine the proportion of CD4+CD25+ Tregs and the expression of Foxp3 mRNA, respectively. Proliferation of T lymphocytes was assayed with MTT reagent, and the level of transforming growth factor 1 (TGF-1) in the supernatant of concanavalin A (Con A)-induced T lymphocytes was determined by ELISA kit. Our investigations demonstrated that inhibition of arthritis by leflunomide was related to changes in CD4+CD25+ Tregs. In addition, A771726, which is the active metabolite of leflunomide, promoted the differentiation of spleen lymphocytes into CD4+CD25+ Tregs, increased antiinflammatory cytokine TGF-1 secretion, and adjusted the activity of Con A-induced lymphocytes in vitro.

La présente étude a examiné l’effet du léflunomide sur les cellules T régulatrices CD4+ et CD25+ (CD4+CD5+ Treg) immunosuppressives chez des rats souffrant d’arthrite induite au collagène (AIC). On a induit l’AIC chez des rats Wistar au moyen de collagène de type II. On a utilisé la cytométrie en flux couplée à l’immunofluorescence et la technique RT-PCR pour déterminer la proportion de CD4+CD5+ Treg et l’expression de l’ARNm de Foxp3, respectivement. On a évalué la prolifération de lymphocytes T par la méthode du MTT. On a déterminé, à l’aide d’une trousse Elisa, le taux de TGF-1 dans le surnageant des lymphocytes T induits par Con A. Nos examens ont démontré que l’inhibition de l’arthrite par le léflunomide était associée à des modifications dans les cellules CD4+CD5+ Treg. De plus, le métabolite actif du léflunomide, A771726, a facilité la différenciation des lymphocytes spléniques dans les CD4+CD5+ Treg, augmenté la sécrétion des cytokines anti-inflammatoires TGF-1 et ajusté l’activité des lymphocytes induits par CON A.

Document Type: Research Article

Publication date: January 1, 2010

More about this publication?
  • Published since 1929, this monthly journal reports current research in all aspects of physiology, nutrition, pharmacology, and toxicology, contributed by recognized experts and scientists. It publishes symposium reviews and award lectures and occasionally dedicates entire issues or portions of issues to subjects of special interest to its international readership.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more