Authors: Monge, Claire; Beraud, Nathalie; Tepp, Kersti; Pelloux, Sophie; Chahboun, Siham; Kaambre, Tuuli; Kadaja, Lumme; Roosimaa, Mart; Piirsoo, Andres; Tourneur, Yves; Kuznetsov, Andrey V.; Saks, Valdur; Seppet, Enn

Source: Canadian Journal of Physiology and Pharmacology, Volume 87, Number 4, April 2009 , pp. 318-326(9)

Publisher: NRC Research Press

Abstract:

Comparative analysis of the bioenergetic parameters of adult rat cardiomyocytes (CM) and HL-1 cells with very different structure but similar cardiac phenotype was carried out with the aim of revealing the importance of the cell structure for regulation of its energy fluxes. Confocal microscopic analysis showed very different mitochondrial arrangement in these cells. The cytochrome content per milligram of cell protein was decreased in HL-1 cells by a factor of 7 compared with CM. In parallel, the respiratory chain complex activities were decreased by 4-8 times in the HL-1 cells. On the contrary, the activities of glycolytic enzymes, hexokinase (HK), and pyruvate kinase (PK) were increased in HL-1 cells, and these cells effectively transformed glucose into lactate. At the same time, the creatine kinase (CK) activity was significantly decreased in HL-1 cells. In conclusion, the results of this study comply with the assumption that in contrast to CM in which oxidative phosphorylation is a predominant provider of ATP and the CK system is a main carrier of energy from mitochondria to ATPases, in HL-1 cells the energy metabolism is based mostly on the glycolytic reactions coupled to oxidative phosphorylation through HK.

On a effectué chez des rats adultes une analyse comparée des paramètres bioénergétique des cardiomyocytes (CM) et des cellules HL-1, qui ont une structure très différente mais un phénotype cardiaque similaire, pour montrer l'importance de la structure cellulaire pour la régulation de ses flux énergétiques. L'analyse par microscopie confocale a montré une répartition mitochondriale différente dans ces cellules. La teneur en cytochromes par mg de protéine cellulaire a diminué d'un facteur 7 dans les cellules HL-1 par comparaison à celles des CM. Parallèlement, les activités complexes de la chaîne respiratoire ont diminué de 4 à 8 fois dans les cellules HL-1. En revanche, les activités des enzymes glycolytiques, hexokinase (HK) et pyruvate kinase (PK), ont augmenté dans les cellules HL-1, lesquelles ont transformé efficacement le glucose en lactate. L'activité de la créatine kinase (CK) des cellules HL-1 a aussi diminué significativement. En conclusion, les résultats de cette étude viennent étayer à l'hypothèse que, contrairement aux CM où la phosphorylation oxydative est un fournisseur important d'ATP et le système CK, le principal transporteur d'énergie des mitochondries vers les ATPases, dans les cellules HL-1, le métabolisme énergétique repose principalement sur les réactions glycolytiques couplées à la phosphorylation oxydative par l'HK.

Document Type: Research article

Publication date: 2009-04-01

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• In this Subject: Anatomy & Physiology ,  Pharmacology
• By this author: Monge, Claire ;  Beraud, Nathalie ;  Tepp, Kersti ;  Pelloux, Sophie ;  Chahboun, Siham ;  Kaambre, Tuuli ;  Kadaja, Lumme ;  Roosimaa, Mart ;  Piirsoo, Andres ;  Tourneur, Yves ;  Kuznetsov, Andrey V. ;  Saks, Valdur ;  Seppet, Enn

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