Authors: Francés, Daniel; Ronco, M. T.; Ochoa, Elena; Alvarez, M. L.u.j..n.; Quiroga, Ariel; Parody, J. P.a.b.l.o.; Monti, Juan; Carrillo, M. C.r.i.s.t.i.n.a.; Carnovale, Cristina E.

Source: Canadian Journal of Physiology and Pharmacology, Volume 85, Number 10, October 2007 , pp. 1047-1051(5)

Publisher: NRC Research Press

Abstract:

The aim of this study was to evaluate the influence of partial hepatectomy prior to cell isolation on hepatocytes in vitro. We characterized the possible changes of various stress oxidative parameters within the first 24h after seeding. Male Wistar rats served as donors. Hepatocytes were isolated by collagenase digestion from either liver of simulated surgery (SH) or from liver 1h after 70% hepatectomy (PH), and the changes in stress parameters were analyzed after 1, 3, 18, and 24 h in culture. At 24h, only hepatocytes from PH maintained significantly increased reactive oxygen species production, oxidized glutathione percentage, and Cu/Zn superoxide dismutase and catalase activities. Our results show that hepatocytes suffer significant cell injury as a result of the isolation procedure, but primary cultured cells from SH metabolically recover from this stress after 18h. After this time, primary culture hepatocytes primed by PH maintain their in vivo-like metabolic activities (increase in both oxidative stress and antioxidant status).

La présente étude a eu pour but d'évaluer l'effet d'une hépatectomie partielle sur des hépatocytes avant l'isolement des cellules in vitro. Nous avons caractérisé les modifications potentielles de certains paramètres du stress oxydatif dans les 24h qui ont suivi l'ensemencement. Des rats Wistar mâles ont servi de donneurs. Nous avons isolé par digestion à la collagénase des hépatocytes de foies provenant d'une chirurgie simulée (SH) ou 1 heure après une hépatectomie de 70% (HP). Nous avons effectué les examens 1, 3, 18, et 24 h après la mise en culture. Après 24h, seuls les hépatocytes de l'HP maintiennent une production d'espèces oxygénées radicalaires, un taux de glutathion oxydé et des activités de la superoxyde dismutase Cu/Zn et de la catalase significativement élevés. Nos résultats montrent que les hépatocytes présentent une lésion cellulaire importante causée par la technique d'isolement, mais que les cellules en culture primaire provenant de la SH retrouvent leur équilibre métabolique après 18 h. Après cette période, les hépatocytes en culture primaire provenant de l'HP maintiennent leurs activités métaboliques de type in vivo (stress oxydatif et statut antioxydant élevés).

Document Type: Research article

Publication date: 2007-10-01

More about this publication?

• Published since 1929, this monthly journal reports current research in all aspects of physiology, nutrition, pharmacology, and toxicology, contributed by recognized experts and scientists. It publishes symposium reviews and award lectures and occasionally dedicates entire issues or portions of issues to subjects of special interest to its international readership.
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Terms & Conditions
• Sample Issue
• Reprints & Permissions
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Anatomy & Physiology ,  Pharmacology
• By this author: Francés, Daniel ;  Ronco, M. T. ;  Ochoa, Elena ;  Alvarez, M. L.u.j..n. ;  Quiroga, Ariel ;  Parody, J. P.a.b.l.o. ;  Monti, Juan ;  Carrillo, M. C.r.i.s.t.i.n.a. ;  Carnovale, Cristina E.

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers