Antioxidative and free radical scavenging effects of ecdysteroids from Serratula strangulata

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The antioxidative and free radical scavenging effects of four ecdysteroids, 20-hydroxyecdysone (E1), 25-deoxy-11,20-dihydroxyecdysone (E2), 24-(2-hydroxyethyl)-20-hydroxyecdysone (E3), and 20-hydroxyecdysone-20,22-monoacetonide (E4), isolated from the Chinese herbSerratula strangulata have been investigated in vitro. These ecdysteroids could protect human erythrocytes against oxidative hemolysis induced by a water-soluble azo initiator 2,2'-azobis(2-amidinopropane hydrochloride) (AAPH). They could also inhibit the peroxidation of rat liver microsomes induced by hydroxyl radicals, as monitored by the formation of thiobarbituric acid reactive substances (TBARS), and prevent radical-induced decrease of membrane fluidity as determined by fluorescence polarization. They reacted with galvinoxyl radicals in homogeneous solution, and the pseudo-first-order rate constants were determined spectrophotometrically by following the disappearance of galvinoxyl radicals. Compounds E1 and (or) E3 were the most active in both antioxidative and radical-scavenging reactions.Key words: ecdysteroid, Serratula strangulata, free radical, erythrocyte, lipid peroxidation, antioxidant.

On a examiné in vitro les effets antioxydatifs et antiradicalaires de quatre ecdystéroïdes, 20-hydroxyecdysone (E1), 25-désoxy-11,20-dihydroxyecdysone (E2), 24-(2-hydroxyéthyl)-20-hydroxyecdysone (E3) et 20-hydroxyecdysone-20,22-monoacétonide (E4), isolés de la plante chinoise Serratula strangulata. Ces ecdystéroïdes pourraient protéger les érythrocytes humains contre l'hémolyse oxydative induite par l'initiateur azo hydrosoluble, chlorhydrate de 2,2'-azobis(2-amidinopropane) (AAPH). Ils pourraient aussi inhiber la peroxydation induite par les radicaux hydroxyl des microsomes hépatiques de rats, tel qu'indiqué par la formation de substances réagissant avec l'acide thiobarbiturique (TBARS), et prévenir la diminution induite par les radicaux de la fluidité membranaire, tel que déterminé en utilisant la polarisation de fluorescence. Ils ont réagi avec le radical galvinoxyl dans une solution homogène, et les constantes de pseudo-premier ordre ont été déterminées par spectrophotométrie en suivant la disparition du radical. Les composés E1 et (ou) E3 sont les plus actifs dans les réactions antioxydatives et antiradicalaires.Mots clés : ecdystéroïde, Serratula strangulata, radical libre, érythrocyte, peroxydation lipidique, antioxydant.[Traduit par la Rédaction]
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