Isolation and characterization of bacteriophages specific to hydrogen-sulfide-producing bacteria
Source: Canadian Journal of Microbiology, Volume 59, Number 1, January 2013 , pp. 39-45(7)
Publisher: NRC Research Press
Abstract:The objectives of this study were to isolate and characterize bacteriophages specific to hydrogen-sulfide-producing bacteria (SPB) from raw animal materials, and to develop a SPB-specific bacteriophage cocktail for rendering application. Meat, chicken offal, and feather samples collected from local supermarkets and rendering processing plants were used to isolate SPB (n = 142). Bacteriophages (n = 52) specific to SPB were isolated and purified from the above samples using 18 of those isolated SPB strains as hosts. The host ranges of bacteriophages against 5 selected SPB strains (Escherichia coli, Citrobacter freundii, and Hafnia alvei) were determined. Electron microscopy observation of 9 phages selected for the phage cocktail revealed that 6 phages belonged to the family of Siphoviridae and 3 belonged to the Myoviridae family. Restriction enzyme digestion analysis with endonuclease DraI detected 6 distinguished patterns among the 9 phages. Phage treatment prevented the growth of SPB for up to 10 h with multiplicity of infection ratios of 1, 10, 100, and 1000 in tryptic soy broth at 30 °C, and extended the lag phase of SPB growth for 2 h at 22 °C with multiplicities of infection of 10, 100, and 1000. These results suggest that the selected bacteriophage cocktail has a high potential for phage application to control SPB in raw animal materials destined for the rendering process.
Keywords: bacteriophages; bactérie productrice de sulfure d'hydrogène; bactériophage; hydrogen sulfide; hydrogen-sulfide-producing bacteria; matière animale crue; raw animal materials; sulfure d'hydrogène
Document Type: Research Article
Affiliations: 1: Department of Biological Sciences, Clemson University, D239 P&A Building, Clemson, SC 29634, USA 2: Department of Food, Nutrition and Packaging Sciences, Clemson University, D239 P&A Building, Clemson, SC 29634, USA 3: Department of Food, Nutrition and Packaging Sciences, Clemson University, D217 P&A Building, Clemson, SC 29634, USA
Publication date: January 24, 2013
- Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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