Influence of Pythium oligandrum on the bacterial communities that colonize the nutrient solutions and the rhizosphere of tomato plants

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Abstract:

The influence exerted by the biocontrol oomycete Pythium oligandrum on the bacterial populations proliferating in the rhizosphere of tomato plants grown in a hydroponic system and in the circulating solutions is studied in the present experiment. Quantitative PCR and single-strand conformation polymorphism were used to investigate the genetic structure and dynamics of the bacterial communities colonizing the root systems and the various circulating solutions. Quantitative PCR assays showed that bacteria heavily colonized the rhizosphere of tomato plants with, however, no significant density changes throughout the cultural season (April–September). Single strand conformation polymorphism fingerprints revealed the occurrence of transient perturbations in the rhizospheric indigenous bacterial communities following P. oligandrum introduction in the root system of plants. This effect was, however, transient and did not persist until the end of the cropping season. Interestingly, the genetic structure of the bacterial microflora colonizing either the roots or the nutrient solutions evolved throughout the cropping season. This temporal evolution occurred whatever the presence and persistence of P. oligandrum in the rhizosphere. Evidence is also provided that bacterial microflora that colonize the root system are different from the ones colonizing the circulating solutions. The relationships between these 2 microflora (at the root and solution levels) are discussed.

Keywords: bacterial communities; biological control; communautés bactériennes; contrôle biologique; culture hors-sol; polymorphisme de conformation simple brin; recirculating solutions; rhizosphere; rhizosphère; single-strand conformation polymorphism; soilless culture; solutions circulantes

Document Type: Research Article

DOI: http://dx.doi.org/10.1139/w2012-092

Affiliations: 1: Université Européenne de Bretagne / Université de Brest, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, ESMISAB, 29280 Plouzané, France. 2: Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, F-33140 Villenave d’Ornon, France. 3: Centre de Recherche en Horticulture, Pavillon Envirotron, 2480, Boulevard Hochelaga, Université Laval, Québec, QC G1V 0A6, Canada.

Publication date: September 20, 2012

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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