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Transferring chromosome DNA fragments from multiple donor cells into a host strain for yeast strain improvement

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Abstract:

Based on a common biological phenomenon — homologous recombination — a novel method was developed by transferring chromosome DNA fragments extracted from multiple donor cells into a host strain. Through this method of transferring DNA fragments, foreign DNA fragments are introduced into one host cell and multiple positive traits from multiple strains may be integrated into the host strain. We first confirmed its feasibility in both prokaryotic and eukaryotic cells by selecting reverse mutants to prototrophy from auxotrophic strains through receiving chromosomal DNA fragments of wild-type parental strains. We then applied this method to Saccharomyces cerevisiae to improve its ethanol and temperature tolerance. We introduced donor chromosome DNA fragments from different S. cerevisiae strains with improvements in ethanol or temperature tolerance into a common strain S. cerevisiae and obtained a strain with much superior ethanol and temperature tolerance. The results showed that the Transferring DNA Fragments method provides a new way for strain breeding.

Keywords: Transferring DNA Fragments method; ethanol tolerance; fragments d’ADN génomique; genomic DNA fragments; homologous recombination; méthode de transfert de fragments d’ADN; recombinaison homologue; temperature tolerance; tolérance à la température; tolérance à l’éthanol

Document Type: Research Article

DOI: http://dx.doi.org/10.1139/w2012-036

Publication date: June 22, 2012

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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