Distinctive heat-shock response of bioleaching microorganism Acidithiobacillus ferrooxidans observed using genome-wide microarray

Authors: Yin, Huaqun; Tang, Min; Zhou, Zhijun; Fu, Xian; Shen, Li; Liang, Yili; Li, Qian; Liu, Hongwei; Liu, Xueduan

Source: Canadian Journal of Microbiology, Volume 58, Number 5, May 2012 , pp. 628-636(9)

Publisher: NRC Research Press

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Abstract:

Temperature plays an important role in the heap bioleaching. The maldistribution of ventilation in the heap leads to local hyperthermia, which does exert a tremendous stress on bioleaching microbes. In this study, the genome-wide expression profiles of Acidithiobacillus ferrooxidans at 40 °C were detected using the microarray. The results showed that some classic proteases like Lon and small heat-shock proteins were not induced, and heat-inducible membrane proteins were suggested to be under the control of σE. Moreover, expression changes of energy metabolism are noteworthy, which is different from that in heterotrophic bacteria upon heat stress. The induced enzymes catalyzed the central carbon metabolism pathway that might mainly provide precursors of amino acids for protein synthesis. These results will deepen the understanding of the mechanisms of heat-shock response on autotrophic bacteria.

Keywords: Acidithiobacillus ferrooxidans; choc thermique; expression sur tout le génome; genome-wide expression; heat shock; heat-shock proteins (HSPs); protéines du choc thermique

Document Type: Research Article

DOI: http://dx.doi.org/10.1139/w2012-023

Affiliations: School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083 Hunan, China.

Publication date: May 13, 2012

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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