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Method for the validation of intraspecific crosses of Saccharomyces cerevisiae strains by minisatellite analysis

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Abstract:

The crossing of Saccharomyces strains by spore conjugation is one of the ways to obtain new starter cultures for the fermentation industry. One of the major difficulties of this practice is the identification of the newly formed hybrids. In this work we describe an effective molecular method for the validation of Saccharomyces intraspecific crosses. The method described is based in the hypothesis that hybrids constructed by spore conjugation contain the sum of the genomes of both parental strains. As a consequence, the conjugation of spores of two yeasts showing different genomic fingerprinting profiles will result in a hybrid culture that will show the sum of both profiles. We demonstrated that the detection of polymorphism in two genes containing minisatellite-like sequences, either SED1 or AGA1, is suitable for this purpose. Using this strategy we were able to validate 15 crosses out of 162 hybridization attempts.

Keywords: Saccharomyces cerevisiae; breeding; croisement; hybrides; hybrids; minisatellite; polymorphism; polymorphisme

Document Type: Research Article

DOI: http://dx.doi.org/10.1139/w11-142

Affiliations: 1: Department of Agriculture and Food Sciences – University of Modena and Reggio Emilia, via Amendola, 2 – 42122 Reggio Emilia, Italy. 2: AZTI-Tecnalia, Food Research Division, Parque Tecnológico de Bizkaia, Astondo Bidea 609, Derio, Spain.

Publication date: March 27, 2012

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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