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Biological control of Botrytis cinerea using the antagonistic and endophytic Burkholderia cepacia Cs5 for vine plantlet protection

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Antifungal activity of the Burkholderia cepacia Cs5 was tested in vitro and in vivo for the control of Botrytis cinerea. Bacterial biomass was significantly improved by the amendment of ZnSO4, Mo7(NH4)6O24, and mannitol to the NBY medium; consequently, the amount of the secreted fungicides was increased. The quantification of B. cinerea inhibition, in liquid and solid conditions, showed an important sensitivity of this fungus to the strain Cs5 fungicides. Microscopic monitoring impact of these fungicides on mycelium structure showed an important increase in their diameter and ramifications in the presence of 0.75% supernatant. For the in vivo application of the strain Cs5, Vitis vinifera plantlets were inoculated with a Cs5 bacterial suspension, then with B. cinerea spores. The plantlets protection was total and durable when these two inoculations were made 3 weeks apart, which is the time for the endophytic bacterium to colonize the plantlets up to the top leaves. This protection is due to Cs5 antagonism and the elicitation of the plantlets self-defense via the root overgrowth.

Keywords: Botrytis cinerea; Burkholderia cepacia; activité antifongique; antifungal activity; endophytism; endophytisme; plantlets protection; protection des plantules

Document Type: Research Article


Affiliations: 1: Equipe « Biopesticides » L.P.A.P., Centre de Biotechnologie de Sfax, Université de Sfax, P.O. Box 1177, S, Tunisie. 2: Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, P.O. Box 2713-Doha, Qatar.

Publication date: November 20, 2011

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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