Occurrence of potentially pathogenic vibrios and related environmental factors in Songkhla Lake, Thailand

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Abstract:

Vibrios are halophilic bacteria that are ubiquitous in marine environments. Their occurrence in tropical lakes has rarely been investigated. In this study, the predominance and diversity of Vibrio spp. was investigated over a 12-month period in a coastal lagoon, Songkhla Lake, in southern Thailand. Water samples were collected at 2 stations in the estuary near Yor Island in Songkhla Lake. The predominant vibrios were detected by a culture-based method, using thiosulfate–citrate–bile salt–sucrose agar and CHROMagar Vibrio. The diversity of Vibrio spp. was evaluated using denaturant density gradient electrophoresis (DGGE). The highest numbers of total vibrios and Vibrio parahaemolyticus in both areas were observed during the summer. There was no significant correlation between the numbers of vibrios, including V. parahaemolyticus, and either the water temperature or plankton density. Variations in Vibrio species were observed with changes in salinity. Vibrio parahaemolyticus and V. cholerae non-O1/non-O139 were detected during the rainy season when the salinity dropped to nearly 0 parts per thousand. In both areas, V. alginolyticus was the most prominent species detected by the culture method, whereas Vibrio parahaemolyticus was detected by DGGE, every month. Other Vibrio spp. of potential public health concern were also detected by the culture method; they included V. vulnificus, V. fluvialis, and V. mimicus.

Keywords: DGGE; Lac Songkhla; Songkhla Lake; V. parahaemolyticus; Yor Island; vibrios; Île Yor

Document Type: Research Article

DOI: http://dx.doi.org/10.1139/w11-084

Affiliations: 1: Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand. 2: The Establishment Project of Faculty of Medical Technology, Prince of Songkla University, Hat Yai, Thailand. 3: Center for Southeast Asian Studies, Kyoto, Japan.

Publication date: November 21, 2011

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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