Growth of Esteya vermicola in media amended with nitrogen sources yields conidia with increased predacity and resistance to environmental stress

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Abstract:

Esteya vermicola, an endoparasitic fungus of pinewood nematode, exhibits great potential as a biological agent against nematodes. In this study to enhance the sporulation, predacity, and environmental resistance of E. vermicola, various nitrogen sources, such as glycine, l-leucine, and ammonium nitrate, were tested. The supplement of glycine and l-leucine had a significant influence on the growth rate of the colony, enhancing colony dry mass by 5-fold more than did ammonium nitrate or the control. Of the nitrogen sources tested, ammonium nitrate and l-leucine promoted sporulation, yielding more than 6 × 106 CFU/g, while glycine enhanced the proportion of lunate spores. Meanwhile, the supplement of nitrogen sources had a significant influence on adhesive rate and mortality rate against Bursaphelenchus xylophilus. Moreover, the supplement of glycine enhanced the survival rate against heat stress by more than 3-fold that of l-leucine, ammonium nitrate, and control. The spores produced in media amended with glycine, l-leucine, and ammonium nitrate had slightly but not significantly higher UV resistance and drought resistance than spores produced without nitrogen sources. These results suggested that the addition of glycine resulted in the production of E. vermicola conidia with increased predacity and resistance to environmental stress that may be more suitable for control of pine wilt disease.

Keywords: Bursaphelenchus xylophilus; Esteya vermicola; mortality rate; resistance; résistance; taux de mortalité

Document Type: Research Article

DOI: http://dx.doi.org/10.1139/w11-068

Affiliations: 1: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon, 305-764, South Korea. 2: Chinese Academy of Forestry, Beijing 100091, People’s Republic of China.

Publication date: October 27, 2011

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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