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Detection of Aspergillus in lung and other tissue samples using the MycAssay Aspergillus real-time PCR kit

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Abstract:

The MycAssay™ Aspergillus real-time PCR kit was tested on tissues from patients with invasive fungal infections. Tissue samples from nine organ transplant recipients and 33 patients with haematological malignancy were from lung (n = 30), skin (n = 4), and others. Samples were preprocessed with proteinase K and lyticase, followed by DNA extraction and real-time PCR. For all samples, the sensitivity of the MycAssay Aspergillus test was 82% and specificity 79% relative to microscopy and 90% and 64%, respectively, compared with Aspergillus culture. The positive predictive value and negative predictive values compared with culture were 69% and 88% and were 88% and 69% compared with microscopy, respectively. The MycAssay Aspergillus test detected tissue invasive infections with Aspergillus fumigatus, Aspergillus flavus, and Aspergillus terreus.

Keywords: Aspergillus flavus; Aspergillus fumigatus; Aspergillus terreus; PCR; infection fongique massive; invasive fungal infection

Document Type: Research Article

DOI: http://dx.doi.org/10.1139/w11-064

Affiliations: 1: Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Fritz-Pregl-Straße 3, 6020 Innsbruck, Austria. 2: Myconostica Ltd., South Court, Sharston Road, Manchester, M22 4SN, UK.

Publication date: September 30, 2011

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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