Gamma irradiation as a useful tool for the isolation of astaxanthin-overproducing mutant strains of Phaffia rhodozyma
Source: Canadian Journal of Microbiology, Volume 57, Number 9, September 2011 , pp. 730-734(5)
Publisher: NRC Research Press
Abstract:Astaxanthin is a carotenoid pigment responsible for the red color of the flesh of many marine animals. There is an increasing interest in the use of astaxanthin in aquaculture, chemical, pharmaceutical, and alimentary industries. Phaffia rhodozyma has been identified as the best biological source of astaxanthin. Mutagenesis was carried out using different doses of gamma irradiation (1.0, 2.0, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, and 7.0 kGy), and 10 mutant colonies (Gam1–Gam10) were obtained. Highly pigmented mutant strains produced astaxanthin at approximately 15 887.5 µg/L dry mass of yeast, whereas the parental strain produced it at 1061.64 µg/g dry mass of yeast. In the thin-layer chromatography analysis, P. rhodozyma JH-82 and Gam1 mutant strain produced the same retention factor (R f) values, but Gam1 showed a higher astaxanthin content than JH-82.
Document Type: Research Article
Affiliations: 1: Department of BioMedical Sciences, Women Research Center, Alzahra University, Tehran, Iran. 2: Agricultural Biotechnology Department, Faculty of Engineering, Imam Khomeini International University, Qazvin, Iran.
Publication date: September 2011
- Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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