If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Early gene response of human brain microvascular endothelial cells to Listeria monocytogenes infection

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

The gene expression of human brain microvascular endothelial cells (HBMEC) in response to 4 h of infection by Listeria monocytogenes was analyzed. Four hours after infection, the expression of 456 genes of HBMEC had changed (p < 0.05). We noted that many active genes were involved in the formyl-methionyl-leucyl-phenylalanine pathway in infected HBMEC. In the upregulated genes, mRNA levels of interleukin-8 and interleukin-15 in infected cells increased according to microarray and real-time reverse transcription – PCR analyses. Since both cytokines are regarded as potent chemotactic factors, the results suggest that HBMEC are capable of recruiting cells of innate and adaptive immune responses during early L. monocytogenes infection.

Keywords: HBMEC; Listeria; PCR en temps réel; central nervous system; expression génique; gene expression; microarray; puce d’ADN; real-time PCR; système nerveux central

Document Type: Research Article

DOI: http://dx.doi.org/10.1139/w11-018

Affiliations: 1: Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA. 2: School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan. 3: Department of Biological Sciences, Purdue University Calumet, Hammond, IN 46323-2094, USA. 4: Department of Mathematics and Statistics, South Dakota State University, Brookings, SD 57007, USA.

Publication date: May 4, 2011

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more