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Genetic diversity and population structure of Clavibacter michiganensis subsp. nebraskensis

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Clavibacter michiganensis subsp. nebraskensis (CMN) is a gram-positive bacterium and an incitant of Goss’s bacterial wilt and leaf blight or “leaf freckles” in corn. A population structure of a wide temporal and geographic collection of CMN strains (n = 131), originating between 1969 and 2009, was determined using amplified fragment length polymorphism (AFLP) analysis and repetitive DNA sequence-based BOX-PCR. Analysis of the composite data set of AFLP and BOX-PCR fingerprints revealed two groups with a 60% cutoff similarity: a major group A (n = 118 strains) and a minor group B (n = 13 strains). The clustering in both groups was not correlated with strain pathogenicity. Group A contained two clusters, A1 (n = 78) and A2 (n = 40), with a linkage of 75%. Group A strains did not show any correlation with historical, geographical, morphological, or physiological properties of the strains. Group B was very heterogeneous and eight out of nine clusters were represented by a single strain. The mean similarity between clusters in group B varied from 13% to 63%. All strains in group B were isolated after 1999. The percentage of group B strains among all strains isolated after 1999 (n = 69) was 18.8%. Implications of the findings are discussed.

Keywords: AFLP; BOX-PCR; DNA fingerprinting; Goss’s wilt; Rep-PCR; corn; empreinte d’ADN; flétrissement de Goss; maïs

Document Type: Research Article


Affiliations: 1: Plant Pathology Department, University of Nebraska-Lincoln, 204 Morrison Hall, Lincoln, NE 68583-0900, USA. 2: Plant Pathology Department, University of Nebraska-Lincoln, 435 Plant Science Hall, Lincoln, NE 68583-0722, USA. 3: Plant Pathology Department, University of Nebraska-Lincoln, 406C Plant Science Hall, Lincoln, NE 68583-0722, USA.

Publication date: May 21, 2011

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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