The Fluorescence Theatre: a cost-effective device using theatre gels for fluorescent protein and dye screening

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Abstract:

Here we report a simple cost-effective device for screening colonies on plates for expression of the monomeric red fluorescent protein mRFP1 and the fluorescent dye Nile red. This device can be built from any simple light source, in our case a Quebec Colony Counter, and cost-effective theatre gels. The device can be assembled in as little as 20 min, and it produces excellent results when screening a large number of colonies.

Keywords: Nile red; colorant fluorescent; criblage phénotypique; fluorescent dye; fluorescent protein; green fluorescent protein; metagenomics; métagénomique; phenotypic screening; protéine fluorescente; protéine fluorescente rouge; protéine fluorescente verte; red fluorescent protein

Document Type: Research Article

DOI: http://dx.doi.org/10.1139/w11-010

Affiliations: Department of Biology, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L 3G1, Canada.

Publication date: April 8, 2011

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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