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Isolation and characterization of a hydrogen- and ethanol-producing Clostridium sp. strain URNW

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Abstract:

Identification, characterization, and end-product synthesis patterns were analyzed in a newly identified mesophilic, anaerobic Clostridium sp. strain URNW, capable of producing hydrogen (H2) and ethanol. Metabolic profiling was used to characterize putative end-product synthesis pathways of the Clostridium sp. strain URNW, which was found to grow on cellobiose; on hexose sugars, such as glucose, sucrose, and mannose; and on sugar alcohols, like mannitol and sorbitol. When grown in batch cultures on 2 g cellobiose·L-1, Clostridium sp. strain URNW showed a cell generation time of 1.5 h, and the major end-products were H2, formate, carbon dioxide (CO2), lactate, butyrate, acetate, pyruvate, and ethanol. The total volumetric H2 production was 14.2 mmol·(L culture)-1 and the total production of ethanol was 0.4 mmol·(L culture)-1. The maximum yield of H2 was 1.3 mol·(mol glucose equivalent)-1 at a carbon recovery of 94%. The specific production rates of H2, CO2, and ethanol were 0.45, 0.13, and 0.003 mol·h-1·(g dry cell mass)-1, respectively. BLAST analyses of 16S rDNA and chaperonin 60 (cpn60) sequences from Clostridium sp. strain URNW revealed a 98% nucleotide sequence identity with the 16S rDNA and cpn60 sequences from Clostridium intestinale ATCC 49213. Phylogenetic analyses placed Clostridium sp. strain URNW within the butyrate-synthesizing clostridia.

L’identification, la caractérisation et les patrons de synthèse des produits ultimes ont été analysés chez une nouvelle souche mésophile anaérobie de Clostridium sp., URNW, capable de produire de l’hydrogène (H2) et de l’éthanol. Le profil métabolique a été utilisé pour caractériser les voies de synthèse des produits ultimes présumés de Clostridium sp. URNW qui pousse sur le cellobiose, ainsi que sur les hexoses comme le glucose, le sucrose, le mannose, et sur les polyols comme le mannitol et le sorbitol. Clostridium sp. URNW a montré un temps de génération de 1,5 h en culture discontinue dans 2 g de cellobiose·L-1, et les produits ultimes principaux étaient l’hydrogène (H2), le formate, le dioxyde de carbone (CO2), le lactate, le butyrate, l’acétate, le pyruvate et l’éthanol. La production volumétrique totale de H2 était de 14,2 mmol·(L de culture)-1 et la production totale d’éthanol était de 0,4 mmol·(L de culture)-1. Le rendement maximal de H2 était de 1,3 mol·(mol d’équivalent de glucose)-1, à un taux de récupération de carbone de 94 %. Les taux spécifiques de production de H2, de CO2, et d’éthanol étaient de 0,45, 0,13 et 0,003 mol·h-1·(g poids sec de cellules)-1, respectivement. Des analyses BLAST de l’ADNr 16S et des séquences de cpn60 de la souche Clostridium sp. URNW ont révélé une identité de séquence de nucléotides de 98 % avec les séquences d’ADNr 16S et de cpn60 de Clostridium intestinale ATCC 49213. Les analyses phylogéniques placent la souche URNW de Clostridium parmi les clostridiums synthétisant le butyrate.

Document Type: Research Article

Publication date: March 1, 2011

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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