An important role for secreted esterase in disease establishment of the wheat powdery mildew fungus Blumeria graminis f. sp. tritici

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Abstract:

The activity of esterase secreted by conidia of wheat powdery mildew fungus, Blumeria graminis f. sp. tritici, was assayed using indoxyl acetate hydrolysis, which generates indigo blue crystals. Mature, ungerminated, and germinating conidia secrete esterase(s) on artificial media and on plant leaf surfaces. The activity of these esterases was inhibited by diisopropyl fluorophosphate, which is selective for serine esterases. When conidia were inoculated on wheat leaves pretreated with diisopropyl fluorophosphate, both appressorial germ tube differentiation and symptom development were significantly impaired, indicating an important role of secreted serine esterases in wheat powdery mildew disease establishment.

L’activité estérase sécrétée par les conidies de Blumeria graminis f. sp. tritici, le champignon responsable de l’oïdium (blanc) du blé, a été mesurée par l’hydrolyse de l’indoxyle acétate qui génère des cristaux bleu indigo. Les conidies matures, non germées, de même que les conidies en germination sécrètent des estérases sur du milieu artificiel et à la surface des feuilles des végétaux. L’activité de ces estérases était inhibée par le diisopropyle fluorophosphate, lequel est sélectif aux sérine estérases. Lorsque les conidies étaient inoculées sur les feuilles de blé prétraitées au diisopropyle fluorophosphate, la différenciation des tubes germinaux des appressoriums et le développement des symptômes étaient significativement réduits, ce qui indique que les sérine estérases secrétées jouent un rôle important dans l’établissement de l’oïdium chez le blé.

Document Type: Research Article

Publication date: March 1, 2011

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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