Skip to main content

Lactobacillus casei as a probiotic in malnourished Giardia lamblia-infected mice: a biochemical and histopathological study

Buy Article:

$50.00 plus tax (Refund Policy)


The study describes the in vivo activity of Lactobacillus casei in malnourished Giardia lamblia-infected BALB/c mice. By experimentation, it was found that daily administration of the probiotic 7 days before inoculation with Giardia trophozoites in malnourished mice efficiently reduced both the severity and duration of giardiasis. More specifically, excretion of Giardia cysts and trophozoites counts were reduced, while faecal lactobacilli counts increased significantly in probiotic-fed malnourished mice, compared with control mice. Interestingly, it was also observed that oral feeding of the probiotic to malnourished mice abrogated all the anthropometric and biochemical anomalies. Histologically, morphological and cellular alteration of microvillus membrane integrity revealed that probiotic administration ameliorated the mucosal damage in malnourished, probiotic-inoculated, Giardia-infected mice compared with the severe microvillus atrophy, œdematous and vacuolated epithelial cells, and ileitis in malnourished Giardia-infected mice. The results clearly show the antigiardial effect of the probiotic in vivo by modulating the gut cells to inhibit the colonization and multiplication of Giardia trophozoites, thus reducing the severity and duration of murine giardiasis.

Cette étude décrit l’activité de Lactobacillus casei in vivo chez des souris BALB/c malnutries et infectées par Giardia lamblia. Expérimentalement, l’administration quotidienne de probiotiques 7 jours avant l’inoculation de trophozoïtes de Giardia aux souris malnutries des réduisait efficacement tant la sévérité que la durée de la lambliase. Plus spécifiquement, l’excrétion de kystes de Giardia et la numération de trophozoïtes étaient réduites alors que les comptes de lactobacilles fécales augmentaient significativement chez les souris malnutries ayant reçu des probiotiques, comparativement aux souris contrôles. Fait intéressant, l’administration orale de probiotiques chez les souris malnutries annulait toutes les anomalies morphologiques et biochimiques. Histologiquement, la modification morphologique et cellulaire de l’intégrité de la membrane des microvillosités a révélé que l’administration de probiotiques atténuait les dommages causées par Giardia aux muqueuses des souris malnutries, alors que les souris malnutries et infectées n’ayant pas reçu de probiotiques présentaient une atrophie sévère des microvillosités, des cellules épithéliales oedémateuses et vaculolées ainsi que des signes d’iléite. Ces résultats démontrent clairement l’effet anti-lambliase des probiotiques in vivo, par la modulation de la capacité des cellules de l’intestin à inhiber la colonisation et la multiplication des trophozoïtes de Giardia, réduisant ainsi la sévérité et la durée de la lambliase chez la souris.

Document Type: Research Article

Publication date: February 1, 2011

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more