Genetic diversity of picoeukaryotes in eight lakes differing in trophic status

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Abstract:

The genetic diversity of picoeukaryotes (0.2-5.0 µm) was investigated in 8 lakes differing in trophic status in Nanjing, China. Denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing of 18S rRNA genes were applied to analyze the picoeukaryotic communities. DGGE analysis showed that among the 8 lakes, the diversity of picoeukaryotes was greatest in the mesotrophic Lake Nan (24 bands) and least in the oligotrophic Lake Qian (12 bands). Cluster analysis of DGGE profiles revealed that the 8 lakes were grouped into 2 distinct clusters. Cluster 1 contained lakes Mochou, Zixia, Huashen, Nan, Pipa, and Qian, while cluster 2 contained lakes Xuanwu and Baijia. Clone libraries were constructed from the mesotrophic Lake Xuanwu and the oligotrophic Lake Zixia, and the 2 libraries were compared using the program LIBSHUFF. This analysis indicated that the picoeukaryotic community composition differed significantly between the 2 lakes (p = 0.001). A total of 25 operational taxonomic units were detected; 18 (62 clones) were related to known eukaryotic groups, while 7 (30 clones) were not affiliated with any known eukaryotic group. Alveolates and stramenopiles were the dominant groups in Lake Xuanwu, while alveolates and chlorophyta predominated in Lake Zixia. Multivariate statistical analysis indicated that the differences in the picoeukaryotic community composition of the 8 lakes might be related to trophic status and top-down regulation by metazooplankton.

La diversité génétique des picoeucaryotes (0,2-5,0 µm) a été évaluée dans 8 lacs de statuts trophiques différents à Nanjing, Chine. Une électrophorèse sur gel en gradient dénaturant (DGGE), et le clonage et le séquençage des gènes d’ARNr 18S ont été utilisés pour analyser les communautés picoeucaryotes. La DGGE a montré que parmi les 8 lacs, la diversité des picoeucaryotes était plus grande dans le Lac Nan mésotrophe (24 bandes) et moindre dans le Lac Qian oligotrophe (12 bandes). L’analyse de grappes de profils en DGGE a révélé que les 8 lacs étaient groupés en 2 grappes distinctes. La grappe 1 comprenait les lacs Mochou, Zixia, Huashen, Nan, Pipa et Qian, alors que la grappe 2 comprenait les lacs Xuanwu et Baijia. Des banques de clones ont été construites à partir des échantillons du Lac Xuanwu mésotrophe et du Lac Zixia oligotrophe, et les 2 banques ont été comparées à l’aide du programme LIBSHUFF. Cette analyse a indiqué que la composition des communautés picoeucaryotes des 2 lacs différait significativement (p = 0,001). Un total de 25 unités taxonomiques opérationelles a été détecté; 18 (62 clones) étaient reliées à des groupes eucaryotes connus alors que 7 (30 clones) n’étaient affiliées à aucun groupe eucaryote connu. Les alvéolés et la straménopiles constituaient les groupes dominants du Lac Xuanwu, alors que les alvéolés et les chlorophytes étaient prédominants dans le Lac Zixia. Une analyse statistique multivariée a indiqué que les différences dans la composition des communautés picoeucaryotes des 8 lacs pourraient être reliées au statut trophique et à la régulation descendante par le métazooplancton.

Document Type: Research Article

Publication date: February 1, 2011

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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