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rDNA internal transcribed spacer sequence analysis of Craterellus tubaeformis from North America and Europe

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Abstract:

The basidiomycete Craterellus tubaeformis (Fries) Quélet is an important widespread ectomycorrhizal basidiomycete found in the Northern Hemisphere. In this study, 12 samples of C. tubaeformis from North America and Europe were analyzed using internal transcribed spacer (ITS) sequences to reveal the correlation between ITS genotypes and geographic locations and to provide molecular evidence for the identification of C. tubaeformis from different habitats in North America and Europe. The analyses identified abundant sequence variations within C. tubaeformis. The length of the ITS region varied from 571 to 640 bp. The proportion of variable sites was 17.6%, and the proportion of parsimony information sites was 16.7%. Phylogenetic analysis showed some correlations between the ITS genotypes and geographic locations of C. tubaeformis; however, some discrepancies between geographical location and affinity were also found. The results indicated that C. tubaeformis from different habitats in North America and Europe underwent genetic drifting and evolved into 2 different species. nrDNA ITS could be a good markers for distinguishing among C. tubaeformis from different habitats, but rational affinity should be determined by associating the available ITS data with other information sources.

Le basidiomycète Craterellus tubaeformis (Fries) Quélet est un ectomycorhize important, répandu dans l’Hémisphère Nord. Dans cette étude, douze échantillons de C. Tubaeformis d’Amérique du Nord et d’Europe ont été analysés par séquençage de l’espaceur interne transcrit (ITS) afin de déterminer s’il existait une corrélation entre les génotypes des ITS et la localisation géographique du champignon, et de fournir des données moléculaires permettant d’identifier C. tubaeformis provenant de différents habitats d’Amérique du Nord et d’Europe. Les analyses ont permis d’identifier des variations de séquences importantes chez C. tubaeformis. La longueur de la région de l’ITS variait de 571 à 640 pb. La proportion de sites variables était de 17,6 % et la proportion de sites d’information de parcimonie était de 16,7 %. L’analyse phylogénique a montré qu’il existait une certaine corrélation entre les génotypes des ITS et la localisation géographique de C. tubaeformis. Cependant, des divergences entre la localisation géographique et l’affinité ont aussi été trouvées. Les résultats ont indiqué que C. tubaeformis provenant de différents habitats d’Amérique du Nord et d’Europe ont subi une dérive génétique et ont évolué en deux espèces différentes. Les ITS de l’ADNnr pourraient constituer de bons marqueurs permettant de distinguer C. tubaeformis provenant de différents habitats, mais l’affinité rationnelle devrait être déterminée en associant les données disponibles sur les ITS à d’autres sources d’information.

Document Type: Research Article

Publication date: January 1, 2011

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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