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Characterization of Pseudomonas aeruginosa fatty acid profiles in biofilms and batch planktonic cultures

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Abstract:

The fatty acid composition of Pseudomonas aeruginosa PAO1 was compared between biofilm and batch planktonic cultures. Strain PAO1 biofilms were able to maintain a consistent fatty acid profile for up to 6 days, whereas strain PAO1 batch planktonic cultures showed a gradual loss of cis-monounsaturated fatty acids over 4 days. Biofilms exhibited a greater proportion of hydroxy fatty acids but a lower proportion of both cyclopropane fatty acids and saturated fatty acids (SAFAs). SAFAs with >=16 carbons, in particular, decreased in biofilms when compared with that in batch planktonic cultures. A reduced proportion of SAFAs and a decline in overall fatty acid chain length indicate more fluidic biophysical properties for cell membranes of P. aeruginosa in biofilms. Separating the biofilms into 2 partitions and comparing their fatty acid compositions revealed additional trends that were not observed in the whole biofilm: the shear-nonremovable layer consistently showed greater proportions of hydroxy fatty acid than the bulk liquid + shear-removable portion of the biofilm. The shear-nonremovable portion demonstrated a relatively immediate decline in the proportion of monounsaturated fatty acids between days 2 and 4; which was offset by an increase in the proportion of cyclopropane fatty acids, specifically 19:0cyc(11,12). Simultaneously, the shear-removable portion of the biofilm showed an increase in the proportion of trans-monounsaturated fatty acids and cyclopropane fatty acids.

Une comparaison de la composition en acides gras de Pseudomonas aeruginosa PAO1 maintenu en culture planctonique en lot ou en biofilm a été réalisée. Les biofilms de PAO1 maintenaient un profil en acides gras stable pendant 6 jours, alors que les cultures planctoniques en lot de PAO1 perdaient graduellement des acides gras cis-monoinsaturés au cours des quatre premiers jours. Les biofilms contenaient davantage d’acides gras hydroxylés et une proportion plus faible d’acides gras cyclopropaniques et d’acides gras saturés. La quantité d’acides gras saturés de 16 carbones ou plus diminuait dans les biofilms comparativement aux cultures planctoniques en lot. La proportion réduite d’acides gras saturés et la diminution de la longueur de la chaine d’acides gras en général indique que les membranes cellulaires de P. aeruginosa en biofilm sont plus fluides. La séparation des biofilms en deux phases et la comparaison de leur composition en acides gras a révélé d’autres tendances qui n’étaient pas observées dans les biofilms entiers : la couche non détachable par cisaillement possédait systématiquement des concentrations plus élevées d’acides gras hydroxylés comparativement à la portion détachable par cisaillement ou la phase liquide. La proportion d’acides gras monoinsaturés de la portion non détachable par cisaillement déclinait presqu’immédiatement entre les jours 2 et 4, ce qui était compensé par une augmentation de la proportion d’acides gras cyclopropaniques, spécifiquement le 19 :0 cyc(11,12). Simultanément, la proportion d’acides gras trans-monoinsaturés et d’acides gras cyclopropaniques augmentait dans la portion du biofilm détachable par cisaillement.

Document Type: Research Article

Publication date: 2010-12-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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