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Passive protection of purified yolk immunoglobulin administered against Shiga toxin 1 in mouse models

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Shiga toxins produced by Escherichia coli O157:H7 cause a wide spectrum of enteric diseases, such as lethal hemorrhagic colitis and hemolytic uremic syndrome. In this study, the B subunit protein of Shiga toxin type 1 (Stx1) was produced in the E. coli system, was further purified by Ni-column Affinity Chromatography method, and was then used as an immunogen to immunize laying hens for yolk immunoglobulin (IgY) production. Titers of IgY increased gradually with boosting vaccination and, finally, reached a level of 105, remaining steady over 1 year. Then the protective efficacy of IgY against Stx1 was evaluated by in vitro and in vivo experiments. It was shown that the anti-Stx1 IgY could effectively block the binding of Stx1 to the Hela cells and could protect BALB/c mice from toxin challenges. The data indicates the facility of using egg yolk IgY as a therapeutic intervention in cases of Shiga toxin intoxication.

Les toxines Shiga produites par Escherichia coli O157 :H7 causent un large spectre de maladies entériques comme la colite hémorragique létale et le syndrome urémique hémolytique. Dans cette étude, la sous-unité B de la toxine Shiga de type 1 (Stx1) a été produite dans le système E. coli, purifiée par chromatographie d’affinité sur colonne de Ni, et utilisée comme immunogène pour immuniser des poules pondeuses afin d’obtenir des immunoglobulines de jaune d’oeuf (IgY). Les titres en IgY augmentaient graduellement à la suite d’immunisations de rappel, pour atteindre finalement un niveau de 105 stable durant 1 an. Par la suite, l’efficacité protectrice des IgY contre Stx1 a été évaluée in vitro et in vivo. Il a été démontré que les IgY anti-Stx1 bloquaient efficacement la liaison de Stx1 aux cellules HeLa et protégeaient les souris BALB/c lors d’une provocation à la toxine. Les données indiquent qu’il est facile d’utiliser les immunoglobulines de jaune d’oeuf IgY lors d’interventions thérapeutiques dans les cas d’intoxication par les toxines Shiga.

Document Type: Research Article

Publication date: December 1, 2010

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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