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Hemolytic phospholipase Rv0183 of Mycobacterium tuberculosis induces inflammatory response and apoptosis in alveolar macrophage RAW264.7 cells

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The metabolic pathway of phospholipids is one of the most important physiologic pathways in Mycobacterium tuberculosis, a typical intracellular bacterium. The hemolytic phospholipase lip gene (Rv0183) is one of 24 phospholipase genes that have been demonstrated to play critical roles in the metabolism of phospholipids in M. tuberculosis. Quantitative RT-PCR and flow cytometry were used to elucidate the immunological and pathogenic implications of the Rv0183 gene on the inflammatory response following persistent expression of Rv0183 in mouse alveolar macrophage RAW264.7 cells. Our results demonstrate that a time-course-dependent ectopic expression of Rv0183 significantly elevated the expression of IL-6, NF-B, TLR-2, TLR-6, TNFα, and MyD88 in these alveolar macrophage cells. Furthermore, the persistent expression of Rv0183 induced RAW264.7 cell apoptosis in vitro. These findings demonstrate that the expression of Rv0183 induces an inflammatory response and cell apoptosis in the host cells, suggesting that Rv0183 may play an important role in the virulence and pathogenesis of M. tuberculosis infection.

La voie métabolique des phospholipides est une des plus importantes voies physiologiques de Mycobacterium tuberculosis, une bactérie intracellulaire typique. Le gène de la phospholipase hémolytique Lip (Rv0183) est un des 24 gènes codant des phospholipases dont on a démontré qu’il jouait un rôle important dans le métabolisme des phospholipides chez M. tuberculosis. La RT-PCR quantitative et la cytométrie de flux ont été utilisées pour élucider les implications immunologiques et pathogéniques du gène Rv0183 dans la réponse inflammatoire, à la suite de l’expression persistante de Rv0183 dans les macrophages alvéolaires de souris RAW264.7. Nos résultats démontrent qu’en fonction du temps, l’expression ectopique de Rv0183 augmentait significativement l’expression d’IL-6, NF-B, TLR-2, TLR-6, TNFα et MyD88 dans ces macrophages alvéolaires. De plus, l’expression persistante de Rv0183 induisait l’apoptose des cellules RAW264.7 in vitro. Ces résultats démontrent que l’expression de Rv0183 induit la réponse inflammatoire et l’apoptose chez les cellules hôtes, ce qui suggère que Rv0183 joue un rôle important dans la virulence et la pathogenèse de l’infection à M. tuberculosis.

Document Type: Research Article

Publication date: 2010-11-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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