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Selection of genetically diverse Trichoderma spp. isolates for suppression of Phytophthora capsici on bell pepper

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Abstract:

Environmentally compatible control measures are needed for suppression of Phytophthora capsici on pepper. Twenty-three isolates of Trichoderma were screened for suppression of a mixture of 4 genetically distinct isolates of this pathogen on bell pepper (Capsicum anuum) in greenhouse pot assays. Of these 23 isolates, GL12, GL13, and Th23 provided significant suppression of P. capsici in at least 2 assays. These isolates were then compared with Trichoderma virens isolates GL3 and GL21 for suppression of this disease in the presence and absence of the harpin-based natural product Messenger. Isolates GL3 and Th23 provided significant disease suppression (P ≤ 0.05) in 3 of 4 assays, while GL12, GL13, and GL21 provided significant suppression in 2 of 4 assays. There was no apparent benefit from the application of Messenger. Phylogenetic analysis of these 5 isolates (based on the ITS1 region of the nuclear rDNA cluster and tef1), and an additional 9 isolates that suppressed P. capsici in at least 1 assay, separated isolates into 2 clades, with 1 clade containing GL3, GL12, GL13, and GL21. There were also 2 more distantly related isolates, one of which was Th23. We report here the identification of genetically distinct Trichoderma isolates for potential use in disease management strategies employing isolate combinations directed at suppression of P. capsici on pepper.

Des mesures de contrôle compatibles avec l’environnement sont requises pour enrayer la contamination du poivron par Phytophthora capsici. Vingt-trois isolats de Trichoderma ont été criblés quant à leur capacité à enrayer un mélange de 4 isolats génétiquement distincts du pathogène sur le poivron (Capsicum anuum), lors de tests en serre. De ces 23 isolats, GL12, GL13 et Th23 enrayaient significativement P. capsici dans au moins 2 tests. Ces isolats ont alors été comparés aux isolats GL3 et GL21 de Trichoderma virens quant à leur potentiel de suppression de cette maladie en présence ou en absence de Messenger, un produit naturel à base d’harpine. Les isolats GL3 et Th23 inhibaient significativement (P ≤ 0.05) la maladie dans 3 des 4 analyses alors que GL12, GL13, et GL21 l’inhibaient significativement dans 2 des 4 analyses. Il n’y avait pas de bénéfice apparent à l’application de Messenger. L’analyse phylogénique de ces 5 isolats (basée sur la région ITS1 de la grappe d’ADNr nucléaire et de tef1) et de 9 isolats supplémentaires qui supprimaient la croissance de P. capsici dans au moins 1 analyse, a permis de séparer les isolats en 2 clades, 1 clade contenant GL3, GL12, GL13 et GL21. Il y avait aussi 2 isolats reliés mais plus distants, un de ceux-ci étant Th23. Nous rapportons ici l’identification d’isolats génétiquement distincts de Trichoderma potentiellement utilisables dans des stratégies de gestion de la maladie faisant appel à des combinaisons d’isolats visant à enrayer P. capsici sur le poivron.

Document Type: Research Article

Publication date: October 1, 2010

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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