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Effect of diallyldisulphide on an antioxidant enzyme system in Candida species

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Abstract:

This study was carried out to show the effect of diallyldisulphide (DADS), an important organosulphur compound found in garlic (Allium sativum), on antioxidant systems in Candida species. Changes in antioxidant metabolites and antioxidant activity in the presence of DADS were found in Candida albicans and Candida tropicalis. Candida cells were treated with sublethal concentrations of DADS. DADS caused a decrease in the activity of all antioxidant enzymes except catalase, resulting in oxidative stress and damaged cells. The amount of oxidative stress generated by DADS was found to be a function of its concentration. A significant decrease in superoxide dismutase, glutathione-S-transferase, and glutathione peroxidase activities but an increase in catalase activity were observed. Increased levels of lipid peroxidation and decreased levels of glutathione were observed in treated cells. Activity of glucose-6-phosphate dehydrogenase decreased significantly following DADS treatment and could be correlated with a decrease in glutathione concentration in both Candida species. These results indicate that diallyl disulphide acts as a pro-oxidant to Candida species and hence may act as a potent antifungal in the management of candidiasis.

Cette étude a été réalisée pour démontrer l’effet du diallyldisufide (DADS), un composé organosoufré important trouvé dans l’ail (Allium sativum), sur les systèmes antioxydants de Candida. Des changements dans la composition des métabolites et une activité antioxydante ont été trouvés chez Candida albicans et Candida tropicalis mis en présence de DADS. Candida a été traité avec des concentrations sublétales de DADS. Le DADS induisait une diminution de l’activité des enzymes antioxydantes à l’exception de la catalase, causant ainsi un stress oxydant et endommageant les cellules viables. La quantité de stress oxydant généré par le DADS était fonction de sa concentration. Une diminution significative de l’activité de la superoxyde dismutase, de la glutathion-S-transférase et de la glutathion peroxydase était observée, mais l’activité de la catalase était augmentée. Une augmentation des niveaux de peroxydation lipidique et une diminution des niveaux de glutathion ont été observées chez les cellules traitées. L’activité de la glucose-6-phosphate déshydrogénase diminuait significativement à la suite d’un traitement au DADS et cette diminution était corrélée avec la diminution de la concentration de glutathion chez les 2 espèces de Candida. Ces résultats indiquent que le diallyldisulfide agit comme pro-oxydant chez Candida et pourrait ainsi agir comme antifongique dans le traitement de la candidose.

Document Type: Research Article

Publication date: 2010-10-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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