FTIR spectroscopic discrimination of Saccharomyces cerevisiae and Saccharomyces bayanus strains

Authors: Adt, Isabelle; Kohler, Achim; Gognies, Sabine; Budin, Julien; Sandt, Christophe; Belarbi, Abdelkader; Manfait, Michel; Sockalingum, Ganesh D.

Source: Canadian Journal of Microbiology, Volume 56, Number 9, September 2010 , pp. 793-801(9)

Publisher: NRC Research Press

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Abstract:

In this study, we tested the potential of Fourier-transform infrared absorption spectroscopy to screen, on the one hand, Saccharomyces cerevisiae and non-S. cerevisiae strains and, on the other hand, to discriminate between S. cerevisiae and Saccharomyces bayanus strains. Principal components analysis (PCA), used to compare 20 S. cerevisiae and 21 non-Saccharomyces strains, showed only 2 misclassifications. The PCA model was then used to classify spectra from 14 Samos strains. All 14 Samos strains clustered together with the S. cerevisiae group. This result was confirmed by a routinely used electrophoretic pattern obtained by pulsed-field gel electrophoresis. The method was then tested to compare S. cerevisiae and S. bayanus strains. Our results indicate that identification at the strain level is possible. This first result shows that yeast classification and S. bayanus identification can be feasible in a single measurement.

Cette étude visait à tester le potentiel d’utilisation de la spectroscopie IRTF pour cribler d’une part les souches Saccharomyces cerevisiae et non-S. cerevisiae et, d’autre part, pour discriminer les souches S. cerevisiae et Saccharomyces bayanus. L’analyse des composantes principales (ACP), utilisée pour comparer 20 souches de S. cerevisiae et 21 souches non-S. cerevisiae, n’a montré que 2 mauvaises classifications. Le modèle d’APC a été ensuite utilisé pour classer un spectre de 14 souches Samos. Les 14 souches Samos s’aggloméraient avec le groupe S. cerevisiae. Ce résultat a été confirmé par le patron de migration en électrophorèse sur gel en champ pulsé utilisé de routine. Cette méthode a ensuite été testée pour comparer les souches S. cerevisiae et S. bayanus. Nos résultats indiquent que l’identification à l’échelle de la souche est possible. Ce premier résultat montre que la classification des levures et l’identification de S. bayanus est réalisable en une seule analyse.

Document Type: Research Article

Publication date: September 1, 2010

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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